The effect of intratesticular dexpanthenol on experimentally-induced testicular ischaemia/reperfusion injury
Göster/ Aç
Erişim
info:eu-repo/semantics/embargoedAccessTarih
2021Yazar
Aydın, ArifSönmez, Mehmet Giray
Ecer, Gökhan
Kılınç, Fahriye
Kocabaş, Rahim
Atılgan, Adeviye Elçi
Oltulu, Pembe
Balasar, Mehmet
Üst veri
Tüm öğe kaydını gösterKünye
Aydın, A., Sönmez, M. G., Ecer, G., Kılınç, F., Kocabaş, R., Atılgan, A. E. ... Balasar, M. (2021). The effect of intratesticular dexpanthenol on experimentally-induced testicular ischaemia/reperfusion injury. Journal of Pediatric Urology, 17(4), 440.e1-440.e7. https://dx.doi.org/10.1016/j.jpurol.2021.03.031Özet
Introduction
Testis torsion is a urological emergency and a serious situation that may cause testis atrophy, testicular dysfunction and infertility due to ischaemia/reperfusion(I/R) injury even with early intervention.
Objective
To assess the protective effect of dexpanthenol administered intratesticular after detorsion against testis I/R injury.
Study design
Twenty-seven rats were randomly divided into 3 groups containing 9 rats each. The 1st group comprised the sham group with no procedure performed. The 2nd group had only torsion applied, while the 3rd group had torsion + dexpanthenol applied. Rats had 720 degrees clockwise rotation applied to the left testis. After 2 h of ischaemia, testes were de-torsioned and the dexpanthenol group had 500 mg/kg dexpanthenol administered intratesticular after detorsion. After 4 h of reperfusion, rats had blood samples taken and orchiectomy was performed for histologic assessment.
Results
A significant difference was detected in all parameters [necrosis(p:<0.001), Cosentino grade (p < 0.001), congestion (p:0.005), fibrosis (p:<0.001), interstitial oedema (p:0.017), JTBS score (p:<0.001), apoptosis (p < 0.001) and testosterone levels (p:0.006)] when the sham, torsion, and torsion + dexpanthenol groups were compared. Significant differences were observed for fibrosis (p:0.010), Cosentino score (p < 0.001), JTBS score (p:<0.001), apoptosis (p:0.001) and total testosterone levels (p:0.013) when torsion and torsion + dexpanthenol groups were compared. The torsion + dexpanthenol group was identified to have more preservation of testis function observed histologically and hormonally compared to the torsion group.
Discussion
Dexpanthenol is used in many areas due to both epithelizing and antioxidant effects and lack of clear side effects. In spite of use of many chemical and biological agents to protect against testis I/R injury, none have entered routine use.
This study showed that dexpanthenol, which can be easily injected intratesticular during detorsion surgery, has protective effect against histological and functional injury that may develop linked to testis I/R injury. The main limitations of the study are short duration of follow-up due to being a rat experiment and lack of comparison of lipid peroxidation products.
Conclusion
This study identified that dexpanthenol with clinically easy use by intratesticular injection after detorsion during surgery had a protective effect against histological and functional injury that will develop linked to I/R injury in the testis.
WoS Q Kategorisi
Q3Scopus Q Kategorisi
Q2Kaynak
Journal of Pediatric UrologyCilt
17Sayı
4Koleksiyonlar
- Makale Koleksiyonu [3651]
- PubMed İndeksli Yayınlar Koleksiyonu [4050]
- Scopus İndeksli Yayınlar Koleksiyonu [6286]
- WoS İndeksli Yayınlar Koleksiyonu [6433]