dc.contributor.author | Kolbaşı, Bircan | |
dc.contributor.author | Bülbül, Muhammet Volkan | |
dc.contributor.author | Karabulut, Seda | |
dc.contributor.author | Erdem Altun, Ceren | |
dc.contributor.author | Çakıcı, Çağrı | |
dc.contributor.author | Ülfer, Gözde | |
dc.contributor.author | Müdok, Tangül | |
dc.contributor.author | Keskin, İlknur | |
dc.date.accessioned | 2021-05-27T06:49:05Z | |
dc.date.available | 2021-05-27T06:49:05Z | |
dc.date.issued | 2021 | en_US |
dc.identifier.citation | Kolbaşı, B., Bülbül, M. V., Karabulut, S., Erdem Altun, C., Çakıcı, Ç., Ülfer, G. ... Keskin, İ. (2021). Chronic unpredictable stress disturbs the blood-testis barrier affecting sperm parameters in mice. Reproductive BioMedicine Online, 42(5), 983-995. https://dx.doi.org/10.1016/j.rbmo.2020.12.007 | en_US |
dc.identifier.issn | 1472-6483 | |
dc.identifier.issn | 1472-6491 | |
dc.identifier.uri | https://dx.doi.org/10.1016/j.rbmo.2020.12.007 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/6942 | |
dc.description.abstract | Research question: Does chronic stress affect the key proteins and sperm parameters of the blood & ndash;testis barrier (BTB)? Design: C57Bl/6 mice were divided into two groups: a non-treated control group and a chronic unpredictable stress (CUS) applied group. The stress status of the animals was confirmed with behavioural tests. Histopathologic evaluation was conducted by haematoxylin and eosin staining and electron microscope. Malondialdehyde, corticosterone and testosterone levels were evaluated in peripheral blood. Expression levels of BTB proteins, namely zonula occludens-1 (ZO-1), claudin-11 (CLDN11) and clathrin in Sertoli cells, were assessed by Western blotting and immunofluorescence techniques. Sperm samples were collected from cauda epididymis, and sperm parameters analysed. Results: The stress model was confirmed by behavioural tests. Histopathological evaluation of the testes demonstrated a mild degeneration in seminiferous tubules. Malondialdehyde (P = 0.008) and corticosterone levels increased (P = 0.004) and testosterone levels decreased (P = 0.005) in the CUS group. Electron microscopic evaluation confirmed the damage in BTB integrity in the CUS group. Western blot analysis showed that ZO-1 and CLDN11 levels were significantly decreased, although clathrin levels were unchanged. Although sperm concentration and total motility rate were not significantly different between the groups, progressive motility (P = 0.03), normal sperm morphology (P = 0.04), chromatin integrity (toluidine blue) (P = 0.002) and the acrosomal reaction rate (P = 0.002) were significantly decreased, and acrosomal abnormality rate was dramatically increased (P = 0.04) in the CUS group. Conclusions: In mice, CUS disrupted BTB integrity and impaired sperm parameters. A decrease in ZO-1 and CLDN11 expression levels may be proposed as the causative factor. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier Ltd | en_US |
dc.rights | info:eu-repo/semantics/embargoedAccess | en_US |
dc.subject | Blood-Testis Barrier | en_US |
dc.subject | Chronic Unpredictable Stress | en_US |
dc.subject | Sperm | en_US |
dc.subject | Spermatogenesis | en_US |
dc.subject | Testis | en_US |
dc.title | Chronic unpredictable stress disturbs the blood-testis barrier affecting sperm parameters in mice | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Reproductive BioMedicine Online | en_US |
dc.department | İstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER) | en_US |
dc.department | İstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü | en_US |
dc.department | İstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı | en_US |
dc.department | İstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı | en_US |
dc.authorid | 0000-0001-7933-4262 | en_US |
dc.authorid | 0000-0003-1526-2065 | en_US |
dc.authorid | 0000-0003-3302-5004 | en_US |
dc.authorid | 0000-0001-7446-472X | en_US |
dc.authorid | 0000-0002-8662-5284 | en_US |
dc.authorid | 0000-0003-2350-6381 | en_US |
dc.authorid | 0000-0002-7059-1884 | en_US |
dc.identifier.volume | 42 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.startpage | 983 | en_US |
dc.identifier.endpage | 995 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1016/j.rbmo.2020.12.007 | en_US |
dc.identifier.wosquality | Q1 | en_US |
dc.identifier.scopusquality | Q1 | en_US |