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dc.contributor.authorKolbaşı, Bircan
dc.contributor.authorBülbül, Muhammet Volkan
dc.contributor.authorKarabulut, Seda
dc.contributor.authorErdem Altun, Ceren
dc.contributor.authorÇakıcı, Çağrı
dc.contributor.authorÜlfer, Gözde
dc.contributor.authorMüdok, Tangül
dc.contributor.authorKeskin, İlknur
dc.date.accessioned2021-05-27T06:49:05Z
dc.date.available2021-05-27T06:49:05Z
dc.date.issued2021en_US
dc.identifier.citationKolbaşı, B., Bülbül, M. V., Karabulut, S., Erdem Altun, C., Çakıcı, Ç., Ülfer, G. ... Keskin, İ. (2021). Chronic unpredictable stress disturbs the blood-testis barrier affecting sperm parameters in mice. Reproductive BioMedicine Online, 42(5), 983-995. https://dx.doi.org/10.1016/j.rbmo.2020.12.007en_US
dc.identifier.issn1472-6483
dc.identifier.issn1472-6491
dc.identifier.urihttps://dx.doi.org/10.1016/j.rbmo.2020.12.007
dc.identifier.urihttps://hdl.handle.net/20.500.12511/6942
dc.description.abstractResearch question: Does chronic stress affect the key proteins and sperm parameters of the blood & ndash;testis barrier (BTB)? Design: C57Bl/6 mice were divided into two groups: a non-treated control group and a chronic unpredictable stress (CUS) applied group. The stress status of the animals was confirmed with behavioural tests. Histopathologic evaluation was conducted by haematoxylin and eosin staining and electron microscope. Malondialdehyde, corticosterone and testosterone levels were evaluated in peripheral blood. Expression levels of BTB proteins, namely zonula occludens-1 (ZO-1), claudin-11 (CLDN11) and clathrin in Sertoli cells, were assessed by Western blotting and immunofluorescence techniques. Sperm samples were collected from cauda epididymis, and sperm parameters analysed. Results: The stress model was confirmed by behavioural tests. Histopathological evaluation of the testes demonstrated a mild degeneration in seminiferous tubules. Malondialdehyde (P = 0.008) and corticosterone levels increased (P = 0.004) and testosterone levels decreased (P = 0.005) in the CUS group. Electron microscopic evaluation confirmed the damage in BTB integrity in the CUS group. Western blot analysis showed that ZO-1 and CLDN11 levels were significantly decreased, although clathrin levels were unchanged. Although sperm concentration and total motility rate were not significantly different between the groups, progressive motility (P = 0.03), normal sperm morphology (P = 0.04), chromatin integrity (toluidine blue) (P = 0.002) and the acrosomal reaction rate (P = 0.002) were significantly decreased, and acrosomal abnormality rate was dramatically increased (P = 0.04) in the CUS group. Conclusions: In mice, CUS disrupted BTB integrity and impaired sperm parameters. A decrease in ZO-1 and CLDN11 expression levels may be proposed as the causative factor.en_US
dc.language.isoengen_US
dc.publisherElsevier Ltden_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectBlood-Testis Barrieren_US
dc.subjectChronic Unpredictable Stressen_US
dc.subjectSpermen_US
dc.subjectSpermatogenesisen_US
dc.subjectTestisen_US
dc.titleChronic unpredictable stress disturbs the blood-testis barrier affecting sperm parameters in miceen_US
dc.typearticleen_US
dc.relation.ispartofReproductive BioMedicine Onlineen_US
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)en_US
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsüen_US
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalıen_US
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalıen_US
dc.authorid0000-0001-7933-4262en_US
dc.authorid0000-0003-1526-2065en_US
dc.authorid0000-0003-3302-5004en_US
dc.authorid0000-0001-7446-472Xen_US
dc.authorid0000-0002-8662-5284en_US
dc.authorid0000-0003-2350-6381en_US
dc.authorid0000-0002-7059-1884en_US
dc.identifier.volume42en_US
dc.identifier.issue5en_US
dc.identifier.startpage983en_US
dc.identifier.endpage995en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1016/j.rbmo.2020.12.007en_US
dc.identifier.wosqualityQ1en_US
dc.identifier.scopusqualityQ1en_US


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