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dc.contributor.authorDemircan, Turan
dc.contributor.authorSibai, Mustafa
dc.contributor.authorAvşaroğlu, Mahmut Erhan
dc.contributor.authorAltuntaş, Ebru
dc.contributor.authorOvezmyradov, Guvanch
dc.date.accessioned2021-02-11T05:39:17Z
dc.date.available2021-02-11T05:39:17Z
dc.date.issued2021en_US
dc.identifier.citationDemircan, T., Sibai, M., Avşaroğlu, M. E., Altuntaş, E. ve Ovezmyradov, G. (2021). The first report on circulating microRNAs at Pre- and Post-metamorphic stages of axolotls. Gene, 768. https://doi.org/10.1016/j.gene.2020.145258en_US
dc.identifier.issn0378-1119
dc.identifier.issn1879-0038
dc.identifier.urihttps://doi.org/10.1016/j.gene.2020.145258
dc.identifier.urihttps://hdl.handle.net/20.500.12511/6543
dc.description.abstractMicroRNAs (miRNAs) are endogenously coded small RNAs, implicated in post-transcriptional gene regulation by targeting messenger RNAs (mRNAs). Circulating miRNAs are cell-free molecules, found in body fluids, such as blood and saliva, and emerged recently as potential diagnostic biomarkers. Functions of circulating miRNAs and their roles in target tissues have been extensively investigated in mammals, and the reports on circulating miRNAs in non-mammalian clades are largely missing. Salamanders display remarkable regenerative potential, and the Mexican axolotl (Ambystoma mexicanum), a critically endangered aquatic salamander, has emerged as a powerful model organism in regeneration and developmental studies. This study aimed to explore the circulating miRNA signature in axolotl blood plasma. Small RNA sequencing on plasma samples revealed 16 differentially expressed (DE) circulating miRNAs between neotenic and metamorphic stages out of identified 164 conserved miRNAs. Bioinformatics predictions provided functional annotation of detected miRNAs for both stages and enrichment of DE miRNAs in cancer-related and developmental pathways was notable. Comparison with previous reports on axolotl miRNAs unraveled common and unique members of the axolotl circulating miRNome. Overall, this work provides novel insights into non-mammalian aspects of circulating miRNA biology and expands the multi-omics toolkit for this versatile model organism.en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectAxolotlen_US
dc.subjectMetamorphosisen_US
dc.subjectCirculating MiRNAen_US
dc.subjectMiRNAen_US
dc.subjectSmall RNA Sequencingen_US
dc.subjectFunctional Annotationen_US
dc.titleThe first report on circulating microRNAs at Pre- and Post-metamorphic stages of axolotlsen_US
dc.typearticleen_US
dc.relation.journalGeneen_US
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)en_US
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Biyoistatistik ve Tıp Bilişimi Ana Bilim Dalıen_US
dc.authorid0000-0002-1502-1600en_US
dc.identifier.volume768en_US
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/215S526
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1016/j.gene.2020.145258en_US


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