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dc.contributor.authorAbuarqoub, Duaa A.
dc.contributor.authorAslam, Nazneen
dc.contributor.authorJafar, Hanan D.
dc.contributor.authorHarfil, Zakariya Abu
dc.contributor.authorAwidi, Abdalla S.
dc.date.accessioned2020-12-21T08:28:34Z
dc.date.available2020-12-21T08:28:34Z
dc.date.issued2020en_US
dc.identifier.citationAbuarqoub, D. A., Aslam, N., Jafar, H. D., Harfil, Z. A. ve Awidi, A. S. (2020). Biocompatibility of biodentine™ ® with periodontal ligament stem cells: In vitro study. Dentistry Journal, 8(1). https://dx.doi.org/10.3390/dj8010017en_US
dc.identifier.issn2304-6767
dc.identifier.urihttps://dx.doi.org/10.3390/dj8010017
dc.identifier.urihttps://hdl.handle.net/20.500.12511/6110
dc.description.abstractBiodentine™ is a tricalcium silicate-based cement material that has a great impact on different biological processes of dental stem cells, compared to other biomaterials. Therefore, we aimed to investigate the optimum biocompatible concentration of Biodentine™ with stem cells derived from periodontal ligament (hPDLSCs) by determining cell proliferation, cytotoxicity, migration, adhesion and mineralization potential. hPDLSCs were treated with Biodentine™ extract at different concentrations; 20, 2, 0.2 and 0.02 mg/mL. Cells cultured without Biodentine™ were used as a blank control. The proliferation potential of hPDLSCs was evaluated by MTT viability analysis for 6 days. Cytotoxicity assay was performed after 3 days by using AnnexinV/7AAD. Migration potential was investigated by wound healing and transwell migration assays at both cellular and molecular levels. The expression levels of chemokines CXCR4, MCP-1 and adhesion molecules FGF-2, FN, VCAM and ICAM-1 were measured by qPCR. The communication potentials of these cells were determined by adhesion assay. In addition, mineralization potential was evaluated by measuring the expression levels of osteogenic markers; ALP, OCN, OPN and Collagen type1 by qPCR. Our results showed significant increase in the proliferation of hPDLSCs at low concentrations of Biodentine™ (2, 0.2 and 0.02 mg/mL) while higher concentration (20 mg/mL) exhibited cytotoxic effect on the cells. Moreover, 2 mg/mL Biodentine™ showed a significant increase in the migration, adhesion and mineralization potentials of the derived cells among all concentrations and when compared to the blank control. Our findings suggest that 2 mg/mL of Biodentine™ is the most biocompatible concentration with hPDLSCs, showing a high stimulatory effect on the biological processes.en_US
dc.description.sponsorshipCell Therapy Center-The University of Jordanen_US
dc.language.isoengen_US
dc.publisherMDPI Multidisciplinary Digital Publishing Instituteen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectBiocompatibilityen_US
dc.subjectBiodentine™en_US
dc.subjecthPDLSCsen_US
dc.subjectStem Cellsen_US
dc.titleBiocompatibility of biodentine™ ® with periodontal ligament stem cells: In vitro studyen_US
dc.typearticleen_US
dc.relation.journalDentistry Journalen_US
dc.departmentİstanbul Medipol Üniversitesi, Diş Hekimliği Fakültesien_US
dc.identifier.volume8en_US
dc.identifier.issue1en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.3390/dj8010017en_US


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