Show simple item record

dc.contributor.authorAbuarqoub, Duaa A.
dc.contributor.authorAslam, Nazneen
dc.contributor.authorJafar, Hanan D.
dc.contributor.authorHarfil, Zakariya Abu
dc.contributor.authorAwidi, Abdalla S.
dc.identifier.citationAbuarqoub, D. A., Aslam, N., Jafar, H. D., Harfil, Z. A. ve Awidi, A. S. (2020). Biocompatibility of biodentine™ ® with periodontal ligament stem cells: In vitro study. Dentistry Journal, 8(1).
dc.description.abstractBiodentine™ is a tricalcium silicate-based cement material that has a great impact on different biological processes of dental stem cells, compared to other biomaterials. Therefore, we aimed to investigate the optimum biocompatible concentration of Biodentine™ with stem cells derived from periodontal ligament (hPDLSCs) by determining cell proliferation, cytotoxicity, migration, adhesion and mineralization potential. hPDLSCs were treated with Biodentine™ extract at different concentrations; 20, 2, 0.2 and 0.02 mg/mL. Cells cultured without Biodentine™ were used as a blank control. The proliferation potential of hPDLSCs was evaluated by MTT viability analysis for 6 days. Cytotoxicity assay was performed after 3 days by using AnnexinV/7AAD. Migration potential was investigated by wound healing and transwell migration assays at both cellular and molecular levels. The expression levels of chemokines CXCR4, MCP-1 and adhesion molecules FGF-2, FN, VCAM and ICAM-1 were measured by qPCR. The communication potentials of these cells were determined by adhesion assay. In addition, mineralization potential was evaluated by measuring the expression levels of osteogenic markers; ALP, OCN, OPN and Collagen type1 by qPCR. Our results showed significant increase in the proliferation of hPDLSCs at low concentrations of Biodentine™ (2, 0.2 and 0.02 mg/mL) while higher concentration (20 mg/mL) exhibited cytotoxic effect on the cells. Moreover, 2 mg/mL Biodentine™ showed a significant increase in the migration, adhesion and mineralization potentials of the derived cells among all concentrations and when compared to the blank control. Our findings suggest that 2 mg/mL of Biodentine™ is the most biocompatible concentration with hPDLSCs, showing a high stimulatory effect on the biological processes.en_US
dc.description.sponsorshipCell Therapy Center-The University of Jordanen_US
dc.publisherMDPI Multidisciplinary Digital Publishing Instituteen_US
dc.rightsAttribution 4.0 International*
dc.subjectStem Cellsen_US
dc.titleBiocompatibility of biodentine™ ® with periodontal ligament stem cells: In vitro studyen_US
dc.relation.journalDentistry Journalen_US
dc.departmentİstanbul Medipol Üniversitesi, Diş Hekimliği Fakültesien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US

Files in this item


This item appears in the following Collection(s)

Show simple item record

Except where otherwise noted, this item's license is described as info:eu-repo/semantics/openAccess