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Complexities of the chemogenetic toolkit: Differential mDAAO activation by D-amino substrates and subcellular targeting
(Elsevier Inc., 2021)
A common approach to investigate oxidant-regulated intracellular pathways is to add exogenous H2O2 to living cells or tissues. However, the addition of H2O2 to the culture medium of cells or tissues approach does not ...
Chemogenetic tools and genetic biosensors in redox biology: Probing Hyper7 signals in specific cellular locales
(Elsevier Science Inc., 2023)
H2O2 is one of the most studied reactive oxygen species (ROS) in redox biology because of its critical role in oxidant-dependent signaling pathways. Conventional approaches apply exogenous H2O2 on cells and tissues, which ...
Simultaneous manipulation and imaging of chemogenetically induced hydrogen peroxide in hardly transfectable endothelial cells
(Sivas Cumhuriyet University, 2022)
Hydrogen peroxide (H2O2) is a critical signaling molecule in vascular cells, which controls signaling events, yet it can cause pathological oxidative stress in excess. The lack of suitable tools undermined experimental ...