Methodological evaluation of gingival crevicular fluid volume and neutrophil elastase levels: Sequential sampling, length of sampling time and two different sampling methods
AuthorKayar, Nezehat Arzu
Oduncuoğlu, Bahar Füsun
Alptekin, Nilgün Özlem
MetadataShow full item record
CitationKayar, N. A., Oduncuoğlu, B. F., Haliloğlu, S., Serpek, B., Ataoğlu, T. ve Alptekin, N. Ö. (2020). Methodological evaluation of gingival crevicular fluid volume and neutrophil elastase levels: Sequential sampling, length of sampling time and two different sampling methods. Acta Odontologica Scandinavica, 78(4), 290-296. https://doi.org/10.1080/00016357.2019.1703141
Objectives: The mechanisms underlying the formation and composition of gingival crevicular fluid (GCF) and its flow into and from periodontal pockets are not understood very well. The aim of this study was to evaluate the length of sampling time and sequential sampling of GCF neutrophil elastase (NE) enzyme levels by using intracrevicular and orifice methods. Material and methods: Twenty adults (mean age of 41.8 years, ranged 31-60 years, 18 males and 2 females) with chronic periodontitis were enrolled and all completed the 3-d study. GCF was collected by both intracrevicular and intrasulcular methods, 720 samples of GCF were collected. In first, second and third day, the length of sampling time in seconds (s) and order were '5- 10-30-s'; '10- 30- 5-s' and '30- 5- 10-s,' respectively. GCF elastase levels were determined by hydrolysis of neutrophil specific substrate N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide. Results: NE activity (mu U) and NE activity/volume (mu U/mu l) were significantly different for order of sampling (p < .05), but not for the length of sampling time (p>.05). Conclusions: Within the limits of this study, the choice of sampling technique in GCF-profile studies seems to be a critical decision as it has the potential to affect the GCF volume and NE activity.
SourceActa Odontologica Scandinavica
Showing items related by title, author, creator and subject.
Effects of water samples in polyethylene terephthalate bottles stored at different conditions on zebrafish embryos with relevance to endocrine disrupting chemical migration and adenomatous polyposis coli tumor suppressor gene Üstündağ, Ünsal Veli; Ünal, İsmail; Ateş, Perihan Seda; Tırpanç, Aybüke; Eğilmezer, Gizem; Alturfan, Ahmet Ata; Yiğitbaşı, Türkan; Emekli-Alturfan, Ebru (Marmara University, 2019)Objective: Polyethylene terephthalate (PET) is a material that is most commonly used for production of clear plastic bottles. Adenomatous polyposis coli (APC) and beta-catenin have been related with cancer. Aim was to ...
Determination of Se, Cr, Mn, Zn, Co, Na, and K in blood samples of breast cancer patients to investigate their variation using ICP-MS and ICP-OES Toker, Ozan; Topdağı, Ömer; Bakirdere, Sezgin; Bursalıoğlu, Ertuğrul Osman; Öz, Ersoy; Eyecioğlu, Önder; Karabul, Yaşar; Çağlar, Mustafa; İçelli, Orhan (Perkin - Elmer Corp., 2019)The aim of this study was to investigate the concentration changes of Se, Mn, Cr, Zn, Co, Na and K in blood samples of breast cancer patients. Determination of the elements was performed using ICP-MS and ICP-OES instruments. ...
Recombinant plasmid-based quantitative Real-Time PCR analysis of salmonella enterica serotypes and its application to milk samples Gökduman, Kurtuluş; Avşaroğlu, Dilek; Çakiriş, Aris; Üstek, Duran; Gürakan, Candan (Elsevier Science Bv, 2016)The aim of the current study was to develop, a new, rapid, sensitive and quantitative Salmonella detection method using a Real-Time PCR technique based on an inexpensive, easy to produce, convenient and standardized ...