Isolation of anti-inflammatory compounds from Sambucus ebulus leaves through in vitro activity-guided fractionation
İlter, Ayça Zeynep
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CitationAtay, İ., İlter, A. Z., Bagatur, Y., Telci, D., Gören, A., Kırmızıbekmez, H. ve Yeşilada, E. (2015). Isolation of anti-inflammatory compounds from Sambucus ebulus leaves through in vitro activity-guided fractionation. 63rd International Congress and Annual Meeting of the Society-for-Medicinal-Plant-and-Natural-Product-Research içinde (1512-1512. ss.). Budapest, Hungary, August 23-27, 2015. https://dx.doi.org/10.1055/s-0035-1565693
The in vitro anti-inflammatory effects of subextracts (n-hexane, chloroform, ethyl acetate, n-butanol, remaining water) of the methanol extract of the leaves of Sambucus ebulus L. (Adoxaceae) were investigated for their inhibitory activities on the activation of Nuclear factor kappa B (NF-κB) on lipopolysaccharide induced Raw 264.7 cells. The n-hexane, chloroform and ethyl acetate subextracts inhibited NF-κB activation at 50, 100 and 100 µg/mL concentrations, respectively. Two flavonoid mixtures [quercetin-3-O-β-D- glucopyranoside, quercetin-3-O-β-D-galactopyranoside], two flavonoids [isorhamnetin-3-O- β-D-glucopyranoside (1), isorhamnetin-3-O-rutinoside (2)] were isolated from ethyl acetate subextract. 10-O-acetylpatrinoside (3) and a new iridoid [Sambulin B (4)] was obtained from chloroform and n-hexane subextracts respectively. Structures were elucidated by NMR and MS. The compounds exerted inhibitions between 30 – 80% on NF-κB. Flavonoids were applied to cells at 25, 50, 75 and 100 µg/mL concentrations. Sambulin B was applied at 6,25, 12,5, 25 and Sambulin A applied at 12,5, 25 and 50 µg/mL concentrations.