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dc.contributor.authorAtay Balkan, İrem
dc.contributor.authorGören, Ahmet Ceyhan
dc.contributor.authorKırmızıbekmez, Hasan
dc.contributor.authorYeşilada, Erdem
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:50:53Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:50:53Z
dc.date.issued2018en_US
dc.identifier.citationAtay Balkan, İ., Gören, A. C., Kırmızıbekmez, H. ve Yeşilada, E. (2018). Evaluation of the in vitro anti-inflammatory activity of Nerium oleander L. flower extracts and activity-guided isolation of the active constituents. Records of Natural Products, 12(2), 128-141. https://dx.doi.org/10.25135/rnp.15.17.05.100en_US
dc.identifier.issn1307-6167
dc.identifier.urihttps://dx.doi.org/10.25135/rnp.15.17.05.100
dc.identifier.urihttps://hdl.handle.net/20.500.12511/2096
dc.descriptionWOS: 000416971500003en_US
dc.description.abstractThe in vitro anti-inflammatory activity profile of the Nerium oleander flower EtOH extract/its subextracts (n-hexane, CH2Cl2, EtOAc, remaining H2O) were evaluated on LPS induced Raw 264.7 macrophages. The effects of the crude EtOH extract and its subextracts on nitric oxide (NO) production and cell viability were determined. The most active subextract was determined to be the EtOAc subextract without exerting any toxicity towards Raw 264.7 macrophages. This subextract significantly inhibited NO production of Raw 264.7 macrophages after LPS induction (62.56 +/- 1.91% at 200 mu g/mL concentration). The levels of iNOS were reduced up to 67.50%. Moreover, this subextract slightly reduced the phosphorylation levels of MAP kinases (p-ERK, p-JNK, p-38). The highest inhibition was observed for ERK phosphorylation, which was inhibited by 20.53% at 200 mu g/mL concentration. Through activity-guided fractionation procedures, kaempferol, kaempferol 3-O-beta-glucopyranoside and chlorogenic acid were isolated as the main active components. The structures of the active compounds were determined by 2D-NMR techniques and HRMS analysis. All compounds significantly inhibited NO productions. Results of the present study supported the traditional use of N. oleander flowers to treat inflammatory complaints.en_US
dc.language.isoengen_US
dc.publisherACG Publicationsen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectNerium Oleanderen_US
dc.subjectNitric Oxideen_US
dc.subjectMitogen Activated Protein Kinasesen_US
dc.subjectKaempferolen_US
dc.subjectKaempferol 3-O-Beta-Glucopyranosideen_US
dc.subjectChlorogenic Aciden_US
dc.titleEvaluation of the in vitro anti-inflammatory activity of Nerium oleander L. flower extracts and activity-guided isolation of the active constituentsen_US
dc.typearticleen_US
dc.relation.journalRecords of Natural Productsen_US
dc.departmentİstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Farmakognozi Ana Bilim Dalıen_US
dc.authorid0000-0002-7921-8621en_US
dc.identifier.volume12en_US
dc.identifier.issue2en_US
dc.identifier.startpage128en_US
dc.identifier.endpage141en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.25135/rnp.15.17.05.100en_US


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