dc.contributor.author | Cansız, Derya | |
dc.contributor.author | Beler, Merih | |
dc.contributor.author | Eğilmezer, Gizem | |
dc.contributor.author | Işıkoğlu, Semanur | |
dc.contributor.author | Mızrak, Zülal | |
dc.contributor.author | Ünal, İsmail | |
dc.contributor.author | Paker, Selçuk | |
dc.contributor.author | Alturfan, Ahmet Ata | |
dc.contributor.author | Emekli Alturfan, Ebru | |
dc.date.accessioned | 2024-06-28T08:17:37Z | |
dc.date.available | 2024-06-28T08:17:37Z | |
dc.date.issued | 2024 | en_US |
dc.identifier.citation | Cansız, D., Beler, M., Eğilmezer, G., Işıkoğlu, S., Mızrak, Z., Ünal, İ. ... Emekli Alturfan, E. (2024). Radiofrequency electromagnetic field exposure amplifies the detrimental effects of fetal hyperglycemia in zebrafish embryos. European Journal of Biology, 83(1), 77-84. http://dx.doi.org/10.26650/EurJBiol.2024.1467244 | en_US |
dc.identifier.issn | 2602-2575 | |
dc.identifier.uri | http://dx.doi.org/10.26650/EurJBiol.2024.1467244 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/12667 | |
dc.description.abstract | Objective: Radiofrequency electromagnetic field (RF-EMF) exposure during the embryonic period can cause defects in the development of the fetus. The study's aim is to evaluate the effects of RF-EMF on the lipid accumulation, oxidant-antioxidant system parameters, locomotor activities, and gene expressions of insulin and leptin as genes related to insulin resistance in fetal hyperglycemia-induced zebrafish embryos. Materials and Methods: The study exposed zebrafish embryos to RF-EMF (60 min) and glucose (5%) every day until 96 hours post fertilization (hpf). The study measured lipid peroxidation (LPO), superoxide dismutase, nitric oxide (NO), glutathione S-transferase (GST), and glutathione (GSH) levels to observe the oxidative stress status. The study monitored the development of the zebrafish embryos under a microscope, performed a locomotor activity analysis, measured acetylcholinesterase activity, and conducted oil red O staining to determine lipid accumulation. The study used reverse transcription polymerase chain reactions (RT-PCRs) to determine the expressions of ins and lepa by using RT-PCR. Results: Both the glucose and RF-EMF applications decreased locomotor activity and increased the LPO and NO levels as oxidative damage indicators. Applying RF-EMF alone increased GST and GSH levels, while applying RF-EMF and glucose showed a decrease in the antioxidant defense systems. ins expression increased in the glucose and RF-EMF groups, while lepa expression increased in the glucose group and decreased in the RF-EMF group. Conclusion: The harmful effects of hyperglycemia and RF-EMF exposure during the fetal period on embryo development need to be supported by studies to confirm the changes the current study has identified at the gene and protein levels. | en_US |
dc.language.iso | eng | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.rights | Attribution-NonCommercial 4.0 International | * |
dc.subject | Fetal Hyperglycemia | en_US |
dc.subject | Insulin Resistance | en_US |
dc.subject | Radiofrequency Electromagnetic Field | en_US |
dc.subject | Zebrafish Embryos | en_US |
dc.title | Radiofrequency electromagnetic field exposure amplifies the detrimental effects of fetal hyperglycemia in zebrafish embryos | en_US |
dc.type | article | en_US |
dc.relation.ispartof | European Journal of Biology | en_US |
dc.department | İstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı | en_US |
dc.authorid | 0000-0002-6274-801X | en_US |
dc.identifier.volume | 83 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.startpage | 77 | en_US |
dc.identifier.endpage | 84 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.26650/EurJBiol.2024.1467244 | en_US |
dc.institutionauthor | Cansız, Derya | |
dc.identifier.scopus | 2-s2.0-85195784692 | en_US |
dc.identifier.scopusquality | Q4 | en_US |