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dc.contributor.authorCansız, Derya
dc.contributor.authorÜnal, İsmail
dc.contributor.authorSürmen, Mustafa Gani
dc.contributor.authorSürmen, Saime
dc.contributor.authorSezer, Zehra
dc.contributor.authorBeler, Merih
dc.contributor.authorGüzel, Elif
dc.contributor.authorAlturfan, Ahmet Ata
dc.contributor.authorEmekli Alturfan, Ebru
dc.date.accessioned2024-05-29T07:14:22Z
dc.date.available2024-05-29T07:14:22Z
dc.date.issued2024en_US
dc.identifier.citationCansız, D., Ünal, İ., Sürmen, M. G., Sürmen, S., Sezer, Z., Beler, M. ... Emekli Alturfan, E. (2024). Gentisic acid exerts neuroprotective effects in neurotoxin-induced Parkinson's disease model in zebrafish: Cross-talk between pathways related with neurodegeneration in the gut-brain axis. Brain Research, 1836. http://dx.doi.org/10.1016/j.brainres.2024.148952en_US
dc.identifier.issn0006-8993
dc.identifier.urihttp://dx.doi.org/10.1016/j.brainres.2024.148952
dc.identifier.urihttps://hdl.handle.net/20.500.12511/12525
dc.description.abstractGiven that global prevalence of Parkinson's disease (PD) is expected to rise over the next few decades, understanding the mechanisms and causes of PD is critical. With emphasis on gut-brain axis, we sought to assess the impact of gentisic acid (GA), a diphenolic compound generated from benzoic acid, in rotenone (Rot) induced PD model in zebrafish. For thirty days, adult zebrafish were exposed to GA and rotenone. Tox-Track program was used to analyze locomotor behaviors in the control, GA, Rot, and Rot + GA groups. LC-MS/MS was performed in brain and intestinal tissues. Proteome Discoverer 2.4 was used to analyze raw files, peptide lists were searched against Danio rerio proteins. Protein interactions or annotations were obtained from STRING database. Tyrosine hydroxylase (Th) staining was performed immunohistochemically in the brain. PD-related gene expressions were determined by RT-PCR. Lipid peroxidation, nitric oxide, superoxide dismutase, glutathione S-transferase, and acetylcholinesterase were measured spectrophotometrically. Improved locomotor behaviors were observed by GA treatment in Rot group as evidenced by increased average speed, exploration rate, and total distance. 5214 proteins were identified in intestinal tissues, 4114 proteins were identified in brain by LC-MS/MS. Rotenone exposure altered protein expressions related to oxidative phosphorylation in brain and intestines. Protein expressions involved in ferroptis and actin cytoskeleton changed in brain and intestines. Altered protein expressions were improved by GA. GA ameliorated Th-immunoreactivity in brain, improved park2, park7, pink1, and lrrk2 expressions. Our results show that GA may be a candidate agent to be evaluated for its potential protective effect for PD.en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGentisic Aciden_US
dc.subjectGut-Brain Axisen_US
dc.subjectLC-MS/MS Analyzesen_US
dc.subjectParkinson's Diseaseen_US
dc.subjectRotenoneen_US
dc.subjectZebrafishen_US
dc.titleGentisic acid exerts neuroprotective effects in neurotoxin-induced Parkinson's disease model in zebrafish: Cross-talk between pathways related with neurodegeneration in the gut-brain axisen_US
dc.typearticleen_US
dc.relation.ispartofBrain Researchen_US
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalıen_US
dc.authorid0000-0002-6274-801Xen_US
dc.identifier.volume1836en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1016/j.brainres.2024.148952en_US
dc.institutionauthorCansız, Derya
dc.identifier.wosqualityQ3en_US
dc.identifier.wos001235886100001en_US
dc.identifier.scopus2-s2.0-85191877486en_US
dc.identifier.pmid38643930en_US
dc.identifier.scopusqualityQ2en_US


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