dc.contributor.author | Karimkhani, Hadi | |
dc.contributor.author | Shojaolsadati, Paria | |
dc.contributor.author | Yiğitbaşı, Türkan | |
dc.contributor.author | Kolbaşı, Bircan | |
dc.contributor.author | Emekli, Neslin | |
dc.date.accessioned | 2024-05-17T10:44:05Z | |
dc.date.available | 2024-05-17T10:44:05Z | |
dc.date.issued | 2024 | en_US |
dc.identifier.citation | Karimkhani, H., Shojaolsadati, P., Yiğitbaşı, T., Kolbaşı, B. ve Emekli, N. (2024). The effect of calpain inhibitor-I on copper oxide nanoparticle-induced damage and cerebral ischemia-reperfusion in a rat model. Biomedicine and Pharmacotherapy, 174. http://dx.doi.org/10.1016/j.biopha.2024.116539 | en_US |
dc.identifier.issn | 0753-3322 | |
dc.identifier.uri | http://dx.doi.org/10.1016/j.biopha.2024.116539 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/12478 | |
dc.description.abstract | This study aimed to investigate the effects of the calpain inhibitor N-Acetyl-Leu-Leu-norleucinal (ALLN) on neuroapoptotic cell damage caused by Copper Oxide Nanoparticles (CuO-NP) and exacerbation of damage through brain ischemia/reperfusion (I/R) in a rat model. Male Wistar Albino rats (n=80) were divided into eight groups: Control, I/R, CuO-NP, CuO-NP+I/R, I/R+ALLN, CuO-NP+ALLN, CuO-NP+I/R+ALLN, and DMSO. Biochemical markers (MBP, S100B, NEFL, NSE, BCL-2, Cyt-C, Calpain, TNF-α, Caspase-3, MDA, and CAT) were measured in serum and brain tissue samples. Histological examinations (H&E staining), DNA fragmentation analysis (TUNEL) were performed, along with Caspase-3 assessment. The ALLN-treated groups exhibited significant improvements in biochemical markers and a remarkable reduction in apoptosis compared to the damaged groups (CuO-NP and I/R). H&E and Caspase-3 staining revealed damage-related morphological changes and reduced apoptosis in the ALLN-treated group. However, no differences were observed among the groups with TUNEL staining. The findings suggest that ALLN, as a calpain inhibitor, has potential implications for anti-apoptotic treatment, specifically in mitigating neuroapoptotic cell damage caused by CuO-NP and I/R. | en_US |
dc.language.iso | eng | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Anti-Apoptotic Treatment | en_US |
dc.subject | Brain İschemia-Reperfusion (I/R) | en_US |
dc.subject | Calpain Inhibitor | en_US |
dc.subject | Cerebral İschemia-Reperfusion (I/R) | en_US |
dc.subject | Copper Oxide Nanoparticle (Cuo-NP) | en_US |
dc.subject | Rat Model | en_US |
dc.title | The effect of calpain inhibitor-I on copper oxide nanoparticle-induced damage and cerebral ischemia-reperfusion in a rat model | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Biomedicine and Pharmacotherapy | en_US |
dc.department | İstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı | en_US |
dc.department | İstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı | en_US |
dc.authorid | 0000-0002-0675-1839 | en_US |
dc.authorid | 0000-0001-7933-4262 | en_US |
dc.authorid | 0000-0002-0109-5086 | en_US |
dc.identifier.volume | 174 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1016/j.biopha.2024.116539 | en_US |
dc.institutionauthor | Yiğitbaşı, Türkan | |
dc.institutionauthor | Kolbaşı, Bircan | |
dc.institutionauthor | Emekli, Neslin | |
dc.identifier.scopus | 2-s2.0-85190292716 | en_US |
dc.identifier.pmid | 38615610 | en_US |
dc.identifier.scopusquality | Q1 | en_US |