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dc.contributor.authorKarataş, Lokman
dc.contributor.authorTatar, Zeynep
dc.contributor.authorJames, Eddie A.
dc.contributor.authorÇolakoğulları, Mukaddes
dc.date.accessioned2024-05-07T14:02:02Z
dc.date.available2024-05-07T14:02:02Z
dc.date.issued2024en_US
dc.identifier.citationKarataş, L., Tatar, Z., James, E. A. ve Çolakoğulları, M. (2024). Investigating associations between hla-dr genotype, h. pylori infection, and anti-caga iga seropositivity in a turkish gastritis cohort. Genes, 15(3). http://dx.doi.org/10.3390/genes15030339en_US
dc.identifier.issn2073-4425
dc.identifier.urihttp://dx.doi.org/10.3390/genes15030339
dc.identifier.urihttps://hdl.handle.net/20.500.12511/12436
dc.description.abstractHelicobacter pylori (H. pylori) is associated with gastric inflammation and mucosal antibodies against its cytotoxin-associated gene A (CagA) are protective. Vaccine-elicited immunity against H. pylori requires MHC class II expression, indicating that CD4+ T cells are protective. We hypothesized that the HLA-DR genotypes in human populations include protective alleles that more effectively bind immunogenic CagA peptide fragments and susceptible alleles with an impaired capacity to present CagA peptides. We recruited patients (n = 170) admitted for gastroendoscopy procedures and performed high-resolution HLA-DRB1 typing. Serum anti-CagA IgA levels were analyzed by ELISA (23.2% positive) and H. pylori classified as positive or negative in gastric mucosal tissue slides (72.9% positive). Pearson Chi-square analysis revealed that H. pylori infection was significantly increased in DRB1*11:04-positive individuals (p = 0.027). Anti-CagA IgA was significantly decreased in DRB1*11:04 positive individuals (p = 0.041). In contrast, anti-CagA IgA was significantly increased in DRB1*03:01 positive individuals (p = 0.030). For these HLA-DRB1 alleles of interest, we utilized two in silico prediction methods to compare their capacity to present CagA peptides. Both methods predicted increased numbers of peptides for DRB1*03:01 than DRB1*11:04. In addition, both alleles preferred distinctively different CagA 15mer peptide sequences for high affinity binding. These observations suggest that DRB1*11:04 is a susceptible genotype with impaired CagA immunity, whereas DRB1*03:01 is a protective genotype that promotes enhanced CagA immunity.en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rightsPublicDomain*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectAnti-Caga Igaen_US
dc.subjectHelicobacter Pylorien_US
dc.subjectHLA DRB1*03:01en_US
dc.subjectHLA DRB1*11:04en_US
dc.titleInvestigating associations between hla-dr genotype, h. pylori infection, and anti-caga iga seropositivity in a turkish gastritis cohorten_US
dc.typearticleen_US
dc.relation.ispartofGenesen_US
dc.departmentİstanbul Medipol Üniversitesi, Sağlık Bilimleri Enstitüsü, Tıbbi Biyoloji ve Genetik Ana Bilim Dalıen_US
dc.authorid0000-0003-1635-9651en_US
dc.identifier.volume15en_US
dc.identifier.issue3en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.3390/genes15030339en_US
dc.institutionauthorKarataş, Lokman
dc.identifier.wosqualityQ2en_US
dc.identifier.wos001193439200001en_US
dc.identifier.scopus2-s2.0-85188735196en_US
dc.identifier.pmid38540398en_US
dc.identifier.scopusqualityQ2en_US


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