dc.contributor.author | Karataş, Lokman | |
dc.contributor.author | Tatar, Zeynep | |
dc.contributor.author | James, Eddie A. | |
dc.contributor.author | Çolakoğulları, Mukaddes | |
dc.date.accessioned | 2024-05-07T14:02:02Z | |
dc.date.available | 2024-05-07T14:02:02Z | |
dc.date.issued | 2024 | en_US |
dc.identifier.citation | Karataş, L., Tatar, Z., James, E. A. ve Çolakoğulları, M. (2024). Investigating associations between hla-dr genotype, h. pylori infection, and anti-caga iga seropositivity in a turkish gastritis cohort. Genes, 15(3). http://dx.doi.org/10.3390/genes15030339 | en_US |
dc.identifier.issn | 2073-4425 | |
dc.identifier.uri | http://dx.doi.org/10.3390/genes15030339 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/12436 | |
dc.description.abstract | Helicobacter pylori (H. pylori) is associated with gastric inflammation and mucosal antibodies against its cytotoxin-associated gene A (CagA) are protective. Vaccine-elicited immunity against H. pylori requires MHC class II expression, indicating that CD4+ T cells are protective. We hypothesized that the HLA-DR genotypes in human populations include protective alleles that more effectively bind immunogenic CagA peptide fragments and susceptible alleles with an impaired capacity to present CagA peptides. We recruited patients (n = 170) admitted for gastroendoscopy procedures and performed high-resolution HLA-DRB1 typing. Serum anti-CagA IgA levels were analyzed by ELISA (23.2% positive) and H. pylori classified as positive or negative in gastric mucosal tissue slides (72.9% positive). Pearson Chi-square analysis revealed that H. pylori infection was significantly increased in DRB1*11:04-positive individuals (p = 0.027). Anti-CagA IgA was significantly decreased in DRB1*11:04 positive individuals (p = 0.041). In contrast, anti-CagA IgA was significantly increased in DRB1*03:01 positive individuals (p = 0.030). For these HLA-DRB1 alleles of interest, we utilized two in silico prediction methods to compare their capacity to present CagA peptides. Both methods predicted increased numbers of peptides for DRB1*03:01 than DRB1*11:04. In addition, both alleles preferred distinctively different CagA 15mer peptide sequences for high affinity binding. These observations suggest that DRB1*11:04 is a susceptible genotype with impaired CagA immunity, whereas DRB1*03:01 is a protective genotype that promotes enhanced CagA immunity. | en_US |
dc.language.iso | eng | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.rights | PublicDomain | * |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Anti-Caga Iga | en_US |
dc.subject | Helicobacter Pylori | en_US |
dc.subject | HLA DRB1*03:01 | en_US |
dc.subject | HLA DRB1*11:04 | en_US |
dc.title | Investigating associations between hla-dr genotype, h. pylori infection, and anti-caga iga seropositivity in a turkish gastritis cohort | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Genes | en_US |
dc.department | İstanbul Medipol Üniversitesi, Sağlık Bilimleri Enstitüsü, Tıbbi Biyoloji ve Genetik Ana Bilim Dalı | en_US |
dc.authorid | 0000-0003-1635-9651 | en_US |
dc.identifier.volume | 15 | en_US |
dc.identifier.issue | 3 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.3390/genes15030339 | en_US |
dc.institutionauthor | Karataş, Lokman | |
dc.identifier.wosquality | Q2 | en_US |
dc.identifier.wos | 001193439200001 | en_US |
dc.identifier.scopus | 2-s2.0-85188735196 | en_US |
dc.identifier.pmid | 38540398 | en_US |
dc.identifier.scopusquality | Q2 | en_US |