dc.contributor.author | Çakıcı, Çağrı | |
dc.contributor.author | Daylan, Benay | |
dc.contributor.author | Ünlüer, Rüveyde Safiye | |
dc.contributor.author | Emekli Alturfan, Ebru | |
dc.contributor.author | Ayla, Şule | |
dc.contributor.author | Eren Gözel, Hilal | |
dc.contributor.author | Yiğit, Pakize | |
dc.contributor.author | Yavuz Dokgöz, Elif | |
dc.contributor.author | Yiğitbaşı, Türkan | |
dc.date.accessioned | 2024-03-08T07:57:40Z | |
dc.date.available | 2024-03-08T07:57:40Z | |
dc.date.issued | 2024 | en_US |
dc.identifier.citation | Çakıcı, Ç., Daylan, B., Ünlüer, R. S., Emekli Alturfan, E., Ayla, Ş., Eren Gözel, H. ... Yiğitbaşı, T. (2024). LDH-A inhibitor as a remedy to potentiate the anticancer effect of docetaxel in prostate cancer. Journal of Cancer, 15(3), 590-602. https://dx.doi.org/10.7150/jca.86283 | en_US |
dc.identifier.issn | 1837-9664 | |
dc.identifier.uri | https://dx.doi.org/10.7150/jca.86283 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/12348 | |
dc.description.abstract | Increased LDH-A activity promotes tumor growth, migration, invasion, and metastasis. This study aimed to investigate the effects of the combination of LDH-A inhibitor and Docetaxel on apoptosis and epithelial-mesenchymal transition (EMT) in the murine prostate cancer (PCa) model. The prostate cancer murine model was developed subcutaneously in 50 male B57CL/6 mice using the Tramp-C2 prostate cancer cell line. From the tumor tissue samples, apoptosis analysis was performed using TUNEL staining, and EMT was investigated using western blot and qPCR. Hematoxylin-eosin staining (HE) and Periodic acid-Schiff staining were used to histopathologically examine liver and kidney tissues. Lactate levels revealed that the Warburg effect was reversed with the LDH-A inhibitor. Both serum and tumor tissue apoptosis increased, and tumor sizes reduced in PCa+LDH-A inhibitor + Docetaxel treatment groups (p<0.05). The combination of LDH-A inhibitor and Docetaxel inhibited EMT mechanism by causing a decrease in Snail, Slug, Twist, and HIF-1α expressions as well as a decrease in N-cadherin and an increase in E-cadherin levels. Reprogramming glucose metabolism with an LDH-A inhibitor can increase the effectiveness of Docetaxel on apoptosis and metastasis mechanisms in PCa. | en_US |
dc.description.sponsorship | Istanbul Medipol University Scientific Research Projects Unit | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Ivyspring International Publisher | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Apoptosis | en_US |
dc.subject | EMT | en_US |
dc.subject | LDH-A Inhibitor | en_US |
dc.subject | Prostate Cancer | en_US |
dc.subject | Warburg Effect | en_US |
dc.title | LDH-A inhibitor as a remedy to potentiate the anticancer effect of docetaxel in prostate cancer | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Journal of Cancer | en_US |
dc.department | İstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı | en_US |
dc.department | İstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı | en_US |
dc.department | İstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Biyoistatistik ve Tıp Bilişimi Ana Bilim Dalı | en_US |
dc.authorid | 0000-0002-8662-5284 | en_US |
dc.authorid | 0000-0002-8302-1127 | en_US |
dc.authorid | 0000-0002-5919-1986 | en_US |
dc.authorid | 0000-0002-0675-1839 | en_US |
dc.identifier.volume | 15 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.startpage | 590 | en_US |
dc.identifier.endpage | 602 | en_US |
dc.relation.tubitak | info:eu-repo/grantAgreement/TUBITAK/SOBAG/119S850 | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.7150/jca.86283 | en_US |
dc.institutionauthor | Çakıcı, Çağrı | |
dc.institutionauthor | Daylan, Benay | |
dc.institutionauthor | Ünlüer, Rüveyde Safiye | |
dc.institutionauthor | Yiğit, Pakize | |
dc.institutionauthor | Yiğitbaşı, Türkan | |
dc.identifier.wosquality | Q2 | en_US |
dc.identifier.wos | 001167297900004 | en_US |
dc.identifier.scopus | 2-s2.0-85185716416 | en_US |
dc.identifier.pmid | 38213726 | en_US |
dc.identifier.scopusquality | Q2 | en_US |