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dc.contributor.authorSürmen, Mustafa Gani
dc.contributor.authorAlkan Bozkaya, Tijen
dc.contributor.authorAteş, Mehmet Şanser
dc.contributor.authorSürmen, Saime
dc.contributor.authorÇakıcı, Çağrı
dc.contributor.authorPençe, Sadrettin
dc.contributor.authorEmekli, Nesrin
dc.date.accessioned2023-06-07T10:15:23Z
dc.date.available2023-06-07T10:15:23Z
dc.date.issued2023en_US
dc.identifier.citationSürmen, M. G., Alkan Bozkaya, T., Ateş, M. Ş., Sürmen, S., Çakıcı, Ç., Pençe, S. ... Emekli, N. (2023). Large-scale proteomic analysis of patients with type 2 diabetes mellitus and atherosclerosis using a label-free LC-MS/MS approach. Experimed, 13(1), 26-38. https://doi.org/10.26650/experimed.1219362en_US
dc.identifier.issn2630-6050
dc.identifier.urihttps://doi.org/10.26650/experimed.1219362
dc.identifier.urihttps://hdl.handle.net/20.500.12511/11038
dc.description.abstractObjective: Type 2 diabetes mellitus (T2D) is a metabolic disease whose molecular events have not yet been fully clarified. However, next-generation powerful molecular approaches such as mass spectrometry (MS)-based proteomics holds promise. In this study, we aimed to reveal the protein profile of serum samples obtained from patients with T2D and atherosclerotic cardiovascular disease using the high-resolution liquid chromatography (LC)-MS/MS system. Materials and Methods: Immune depletion was performed for the top 12 abundant proteins in 10 μl serum samples taken from individuals. Then, tryptic peptides were obtained from total proteins by applying a digestion protocol. Accordingly, reduction, alkylation, and digestion with trypsin enzyme were carried out, respectively. Tryptic peptides were analyzed in an ultra-high-pressure LC-MS/MS system with a label-free proteomic approach. The raw data were processed using the software program. Results: LC-MS/MS analyses revealed 120 proteins with significant expression changes. Some of these proteins were associated with inflammation, lipid transport, and oxidative stress, which are known to play an important role in T2D and its complications. Conclusion: As a result, LC-MS/MS analyses highlighted the proteins that will provide predictions in the treatment and course of T2D. We believe that validation of these proteins with targeted proteomic approaches in a larger sample in further studies will contribute to the development of clinically usable panels.en_US
dc.language.isoengen_US
dc.publisherIstanbul Universityen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rightsAttribution-NonCommercial 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/*
dc.subjectDiabetesen_US
dc.subjectProteomicsen_US
dc.subjectSerumen_US
dc.subjectMass Spectrometryen_US
dc.subjectAtherosclerosisen_US
dc.titleLarge-scale proteomic analysis of patients with type 2 diabetes mellitus and atherosclerosis using a label-free LC-MS/MS approachen_US
dc.typearticleen_US
dc.relation.ispartofExperimeden_US
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalıen_US
dc.authorid0000-0002-8662-5284en_US
dc.authorid0000-0002-0109-5086en_US
dc.identifier.volume13en_US
dc.identifier.issue1en_US
dc.identifier.startpage26en_US
dc.identifier.endpage38en_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.26650/experimed.1219362en_US
dc.institutionauthorÇakıcı, Çağrı
dc.institutionauthorEmekli, Nesrin
dc.identifier.scopus2-s2.0-85173962083en_US
dc.identifier.trdizinid1177371en_US


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