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dc.contributor.authorÇakan, Orhan
dc.contributor.authorHacıhasanoğlu Çakmak, Neziha
dc.contributor.authorBayramoğlu, Feyza
dc.contributor.authorYiğitbaşı, Türkan
dc.date.accessioned2023-03-22T12:21:00Z
dc.date.available2023-03-22T12:21:00Z
dc.date.issued2023en_US
dc.identifier.citationÇakan, O., Hacıhasanoğlu Çakmak, N., Bayramoğlu, F. ve Yiğitbaşı, T. (2023). The optimal method to measure polyamines in serum by using HPLC fluorescence detector. Acta Pharmaceutica Sciencia, 61(1), 91-106. https://dx.doi.org/10.23893/1307-2080.APS6107en_US
dc.identifier.issn1307-2080
dc.identifier.urihttps://dx.doi.org/10.23893/1307-2080.APS6107
dc.identifier.urihttps://hdl.handle.net/20.500.12511/10661
dc.description.abstractMeasurement of polyamines (putrescine, spermidine and spermine) is important in the monitoring of many metabolic events, as well as in cancer prognosis. However, the lack of method standardization and validation for the measurement of poly-amines is a major obstacle. In this study, sample preparation, gradient program, chromatography column, sample concentration studies were attempted and an analysis method was developed for the detection of polyamines in serum by using HPLC fluorescence detector in order to reduce the difficulties experienced with the existing methodologies. This method was validated analytically in accordance with EMA and FDA’s guidelines. In this study, Waters Nova-Pak C18 A chromatography column with 3.9 mm, 150 mm and 4 µm specifications was used for the first time. Acetonitrile was used instead of methanol. Thanks to the newly developed gradient, peak purity and sufficient peak separation were ensured and accurate, sensitive, reliable and reproducible method was developed and validated.en_US
dc.language.isoengen_US
dc.publisherIstanbul Medipol Universityen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDerivatizationen_US
dc.subjectHPLCen_US
dc.subjectN-Acetyl-L Cysteineen_US
dc.subjectO-Phthalaldehydeen_US
dc.subjectPolyaminesen_US
dc.titleThe optimal method to measure polyamines in serum by using HPLC fluorescence detectoren_US
dc.typearticleen_US
dc.relation.ispartofActa Pharmaceutica Scienciaen_US
dc.departmentİstanbul Medipol Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Laboratuvar Teknikleri Ana Bilim Dalıen_US
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalıen_US
dc.authorid0000-0002-6183-5015en_US
dc.authorid0000-0002-0757-9743en_US
dc.authorid0000-0002-0675-1839en_US
dc.identifier.volume61en_US
dc.identifier.issue1en_US
dc.identifier.startpage91en_US
dc.identifier.endpage106en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.23893/1307-2080.APS6107en_US
dc.institutionauthorHacıhasanoğlu Çakmak, Neziha
dc.institutionauthorBayramoğlu, Feyza
dc.institutionauthorYiğitbaşı, Türkan
dc.identifier.scopus2-s2.0-85149203618en_US
dc.identifier.scopusqualityQ3en_US


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