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dc.contributor.authorGenç, Çetin
dc.contributor.authorTahta, Alican
dc.contributor.authorErdağ, Ece
dc.contributor.authorDolaş, İlyas
dc.contributor.authorŞahin, Selim
dc.contributor.authorKaraöz, Erdal
dc.contributor.authorAras, Yavuz
dc.contributor.authorSabancı, Pulat Akın
dc.date.accessioned2022-12-09T09:10:57Z
dc.date.available2022-12-09T09:10:57Z
dc.date.issued2023en_US
dc.identifier.citationGenç, Ç., Tahta, A., Erdağ, E., Dolaş, İ., Şahin, S., Karaöz, E. ... Sabancı, P. A. (2023). Human-derived hair follicle stem cells and hydrogen sulfide on focal cerebral ischemia model: A comparative evaluation of radiologic, neurobehavioral and immunohistochemical results. Brain Research, 1799. https://doi.org/10.1016/j.brainres.2022.148170en_US
dc.identifier.issn0006-8993
dc.identifier.issn1872-6240
dc.identifier.urihttps://doi.org/10.1016/j.brainres.2022.148170
dc.identifier.urihttps://hdl.handle.net/20.500.12511/10101
dc.description.abstractThe present study investigated the effects of intracerebral human-derived hair follicle stem cells (HFBSCs), whether alone or in combination with hydrogen sulfide (H2S) in a rat model of focal cerebral ischemia. The rats were randomly assigned into 4 groups (n = 10): Control (phosphate buffered saline (PBS)), Group A (at 24 h post-middle cerebral artery occlusion(MCAo), stereotaxic intracerebral, 1,0 × 106, total 10 μL HFBSCs), Group B (3–14 d post-MCAo, intraperitoneal (i.p.), 25 μM/kg/day H2S), Group AB (HFBSCs + H2S). Cranial magnetic resonance images were recorded on postoperative 1st and 28th days. Three dimensional analysis was performed to calculate the infarct volumes. Rotarod and cylinder tests were performed after MCAo and finally all rats were euthanized by cardiac perfusion at 28 days after MCAo for immunohistochemical analysis. The reduction in infarct volumes of rats receiving HFBSC was significant. The cranial infarct volume on the postoperative 28th day was significantly higher in the group in which H2S was administered alone compared to the HFBSC alone group. All animals showed steadily improved spontaneous locomotor activity from day 7 post-MCAo on rotarod test, from day 1 on cylinder test, but showed no significant differences at all times. In all groups, the grading scores of CD34, CD5, CD11b and GFAP immunohistochemical markers did not differ significantly. In conclusion, intracerebral HFBSC treatment after 24 h of ischemic stroke may be an effective way to reduce the cranial infarct volume, whereas H2S treatment alone or in combination with HFBSC may not be sufficient for ischemic brain injury.en_US
dc.description.sponsorshipIU-BAP ; Istanbul University Scientific Research Funden_US
dc.language.isoengen_US
dc.publisherElsevier B.V.en_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectCerebral Ischemiaen_US
dc.subjectHair Follicle Stem Cellen_US
dc.subjectHydrogen Sulfideen_US
dc.subjectMiddle Cerebral Artery Occlusionen_US
dc.titleHuman-derived hair follicle stem cells and hydrogen sulfide on focal cerebral ischemia model: A comparative evaluation of radiologic, neurobehavioral and immunohistochemical resultsen_US
dc.typearticleen_US
dc.relation.ispartofBrain Researchen_US
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Beyin ve Sinir Cerrahisi Ana Bilim Dalıen_US
dc.authorid0000-0003-0467-7521en_US
dc.identifier.volume1799en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1016/j.brainres.2022.148170en_US
dc.institutionauthorTahta, Alican
dc.identifier.wosqualityQ3en_US
dc.identifier.wos001022010400005en_US
dc.identifier.scopus2-s2.0-85142673045en_US
dc.identifier.pmid36410427en_US


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