Browsing by Author "Eroğlu, Emrah"
Now showing items 1-7 of 7
-
Chemogenetic approaches to dissect the role of H2O2 in redox-dependent pathways using genetically encoded biosensors
Ghaffari Zaki, Asal; Erdoğan, Yusuf Ceyhun; Akgül Çağlar, Tuba; Eroğlu, Emrah (Portland Press Ltd, 2022)Chemogenetic tools are recombinant enzymes that can be targeted to specific organelles and tissues. The provision or removal of the enzyme substrate permits control of its biochemical activities. Yeast-derived enzyme D-amino ... -
A co-culture-based multiparametric imaging technique to dissect local H2O2 signals with targeted HyPer7
Seçilmiş, Melike; Altun, Hamza Yusuf; Pilic, Johannes; Erdoğan, Yusuf Ceyhun; Çokluk, Zeynep; Ata, Büşra Nur; Sevimli, Gülşah; Zaki, Asal Ghaffari; Yiğit, Esra Nur; Öztürk, Gürkan; Malli, Roland; Eroğlu, Emrah (MDPI, 2021)Multispectral live-cell imaging is an informative approach that permits detecting biological processes simultaneously in the spatial and temporal domain by exploiting spectrally distinct biosensors. However, the combination ... -
Complexities of the chemogenetic toolkit: Differential mDAAO activation by D-amino substrates and subcellular targeting
Erdoğan, Yusuf Can; Altun, Hamza Yusuf; Seçilmiş, Melike; Ata, Büşra N.; Sevimli, Gülşah; Çokluk, Zeynep; Zaki, Asal Ghaffari; Sezen, Serap; Akgül Çağlar, Tuba; Sevgen, İlker; Steinhorn, Benjamin; Ai, Huiwang; Öztürk, Gürkan; Belousov, Vsevelod V.; Michel, Thomas; Eroğlu, Emrah (Elsevier Inc., 2021)A common approach to investigate oxidant-regulated intracellular pathways is to add exogenous H2O2 to living cells or tissues. However, the addition of H2O2 to the culture medium of cells or tissues approach does not ... -
Nitric oxide biosensor uncovers diminished ferrous iron-dependency of cultured cells adapted to physiological oxygen levels
Sevimli, Gülşah; Smith, Matthew J.; Akgül Çağlar, Tuba; Bilir, Şükriye; Seçilmiş, Melike; Altun, Hamza Y.; Yiğit, Esra N.; Yang, Fan; Keeley, Thomas P.; Malli, Roland; Öztürk, Gürkan; Mann, Giovanni E.; Eroğlu, Emrah (Elsevier B.V., 2022)Iron is an essential metal for cellular metabolism and signaling, but it has adverse effects in excess. The physiological consequences of iron deficiency are well established, yet the relationship between iron supplementation ... -
Nitric oxide biosensor uncovers diminished ferrous iron-dependency of cultured cells adapted to physiological oxygen levels (vol 53, 102319, 2022)
Sevimli, Gülşah; Smith, Matthew J.; Akgül Çağlar, Tuba; Bilir, Şükriye; Seçilmiş, Melike; Altun, Hamza Y.; Yiğit, Esra N.; Yang, Fan; Keeley, Thomas P.; Malli, Roland; Öztürk, Gürkan; Mann, Giovanni E.; Eroğlu, Emrah (Elsevier, 2022)The authors regret that the cited reference list in the online PDF of this article contains a duplicate reference (Ref 34 and incomplete Ref 56). Ref 56 should read as follows. -
The preamble to the free radical biology and medicine virtual special issue on “Targeting genetic biosensors to intracellular signaling pathways”
Eroğlu, Emrah; Graier, Wolfgang F.; Malli, Roland (Elsevier Inc., 2022)Studying biological signaling pathways is key to understanding the various processes within a cell. The science of biological redox regulation is a rapidly growing research area with implications for various disciplines, ... -
Probing subcellular iron availability with genetically encoded nitric oxide biosensors
Sevimli, Gülşah; Alston, Amy E.; Funk, Felix; Flühmann, Beat; Malli, Roland; Graier, Wolfgang F.; Eroğlu, Emrah (MDPI (Multidisipliner Digital Publishing Institute), 2022)Cellular iron supply is required for various biochemical processes. Measuring bioavailable iron in cells aids in obtaining a better understanding of its biochemical activities but is technically challenging. Existing ...
