dc.contributor.author | Kerman, Bilal Ersen | |
dc.contributor.author | Kim, Hyung-joon | |
dc.contributor.author | Padmanabhan, Krishnan | |
dc.contributor.author | Mei, Arianna | |
dc.contributor.author | Georges, Shereen | |
dc.contributor.author | Joens, Matthew | |
dc.contributor.author | Fitzpatrick, James | |
dc.contributor.author | Jappelli, Roberto | |
dc.contributor.author | Chandross, Karen | |
dc.contributor.author | August, Paul | |
dc.contributor.author | Gage, Fred | |
dc.date.accessioned | 10.07.201910:49:13 | |
dc.date.accessioned | 2019-07-10T19:35:40Z | |
dc.date.available | 10.07.201910:49:14 | |
dc.date.available | 2019-07-10T19:35:40Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Kerman, B. E., Kim, H. J., Padmanabhan, K., Mei, A., Georges, S., Joens, M. S. ... Gage, F. (2015). In vitro myelin formation using embryonic stem cells. Development, 142(12), 2213-2225. https://dx.doi.org/10.1242/dev.116517 | en_US |
dc.identifier.issn | 0950-1991 | |
dc.identifier.issn | 1477-9129 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/893 | |
dc.identifier.uri | https://dx.doi.org/10.1242/dev.116517 | |
dc.description.abstract | Myelination in the central nervous system is the process by which oligodendrocytes form myelin sheaths around the axons of neurons. Myelination enables neurons to transmit information more quickly and more efficiently and allows for more complex brain functions; yet, remarkably, the underlying mechanism by which myelination occurs is still not fully understood. A reliable in vitro assay is essential to dissect oligodendrocyte and myelin biology. Hence, we developed a protocol to generate myelinating oligodendrocytes from mouse embryonic stem cells and established a myelin formation assay with embryonic stem cell-derived neurons in microfluidic devices. Myelin formation was quantified using a custom semi-automated method that is suitable for larger scale analysis. Finally, early myelination was followed in real time over several days and the results have led us to propose a new model for myelin formation. | en_US |
dc.description.sponsorship | National Institutes of Health: P30CA014195 National Institutes of Health: P30NS072031 | en_US |
dc.description.sponsorship | National Institutes of Health: P30NS072031 | en_US |
dc.description.sponsorship | National Multiple Sclerosis Society | en_US |
dc.description.sponsorship | Sanofi and Salk Institute Discovery Award | en_US |
dc.description.sponsorship | Christopher & Dana Reeve Foundation | en_US |
dc.description.sponsorship | Leona M. and Harry B. Helmsley Charitable Trust | en_US |
dc.description.sponsorship | Annette Merle-Smith | en_US |
dc.description.sponsorship | Crick-Jacobs Junior Fellowship | en_US |
dc.description.sponsorship | California Institute for Regenerative Medicine | en_US |
dc.description.sponsorship | Waitt Advanced Biophotonics Center | en_US |
dc.description.sponsorship | W.M. Keck Foundation | en_US |
dc.description.sponsorship | National Cancer Institute (NCI) P30 Cancer Center Support Grant | en_US |
dc.description.sponsorship | National Institute of Neurological Disorders and Stroke (NINDS) P30 Neuroscience Center Core Grant | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Company of Biologists | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Automated Quantification | en_US |
dc.subject | Embryonic Stem Cells | en_US |
dc.subject | Live Imaging | en_US |
dc.subject | Myelin | en_US |
dc.subject | Oligodendrocyte | en_US |
dc.title | In vitro myelin formation using embryonic stem cells | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Development | en_US |
dc.department | İstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER) | en_US |
dc.authorid | 0000-0003-1106-3288 | en_US |
dc.identifier.volume | 142 | en_US |
dc.identifier.issue | 12 | en_US |
dc.identifier.startpage | 2213 | en_US |
dc.identifier.endpage | 2225 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1242/dev.116517 | en_US |
dc.identifier.wosquality | Q1 | en_US |
dc.identifier.scopusquality | Q1 | en_US |