dc.contributor.author | Doeppner, Thorsten R. | |
dc.contributor.author | Doehring, Maria | |
dc.contributor.author | Kaltwasser, Britta | |
dc.contributor.author | Majid, Arshad | |
dc.contributor.author | Lin, Fengyan | |
dc.contributor.author | Bahr, Mathias | |
dc.contributor.author | Kılıç, Ertuğrul | |
dc.contributor.author | Hermann, Dirk M. | |
dc.date.accessioned | 10.07.201910:49:13 | |
dc.date.accessioned | 2019-07-10T20:01:50Z | |
dc.date.available | 10.07.201910:49:13 | |
dc.date.available | 2019-07-10T20:01:50Z | |
dc.date.issued | 2017 | en_US |
dc.identifier.citation | Doeppner, T. R., Doehring, M., Kaltwasser, B., Majid, A., Lin, F., Bahr, M. ... Hermann, D. M. (2017). Ischemic post-conditioning induces post-stroke neuroprotection via Hsp70-mediated proteasome inhibition and facilitates neural progenitor cell transplantation. Molecular Neurobiology, 54(8), 6061-6073. https://dx.doi.org/10.1007/s12035-016-0137-3 | en_US |
dc.identifier.issn | 0893-7648 | |
dc.identifier.issn | 1559-1182 | |
dc.identifier.uri | https://dx.doi.org/10.1007/s12035-016-0137-3 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/3461 | |
dc.description | WOS: 000409039000026 | en_US |
dc.description | PubMed ID: 27699598 | en_US |
dc.description.abstract | In view of the failure of pharmacological therapies, alternative strategies promoting post-stroke brain repair are needed. Post-conditioning is a potentially promising therapeutic strategy, which induces acute neuroprotection against ischemic injury. To elucidate longer lasting actions of ischemic post-conditioning, mice were exposed to a 60-min stroke and post-conditioning by an additional 10-min stroke that was induced 10 min after reperfusion onset. Animals were sacrificed 24 h or 28 days post-stroke. Post-conditioning reduced infarct volume and neurological deficits 24 h poststroke, enhancing blood-brain barrier integrity, reducing brain leukocyte infiltration, and reducing oxidative stress. On the molecular level, post-conditioning yielded increased Hsp70 expression, whereas nuclear factor (NF)-kappa B and proteasome activities were decreased. Reduced infarct volume and proteasome inhibition were reversed by Hsp70 knockdown, suggesting a critical role of the Hsp70 proteasome pathway in ischemic post-conditioning. The survival-promoting effects of ischemic post-conditioning, however, were not sustainable as neuroprotection and neurological recovery were lost 28 days post-stroke. Although angioneurogenesis was not increased by post-conditioning, the favorable extracellular milieu facilitated intracerebral transplantation of neural progenitor cells 6 h post-stroke, resulting in persisted neuroprotection and neurological recovery. Thus, post-conditioning might support brain repair processes, but in view of its transient, neuroprotection is unlikely useful as stroke therapy in its current form. | en_US |
dc.description.sponsorship | TUBITAK [2221] | en_US |
dc.description.sponsorship | The study was supported by TUBITAK (grant no. 2221 to TRD). | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Humana Press Inc | en_US |
dc.rights | info:eu-repo/semantics/embargoedAccess | en_US |
dc.subject | Cerebral Ischemia | en_US |
dc.subject | Stroke | en_US |
dc.subject | Neural Progenitor Cells | en_US |
dc.subject | Post-Conditioning | en_US |
dc.subject | Preconditioning | en_US |
dc.subject | Transplantation | en_US |
dc.subject | Hsp70 | en_US |
dc.subject | Proteasome | en_US |
dc.title | Ischemic post-conditioning induces post-stroke neuroprotection via Hsp70-mediated proteasome inhibition and facilitates neural progenitor cell transplantation | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Molecular Neurobiology | en_US |
dc.department | İstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER) | en_US |
dc.authorid | 0000-0001-6494-8923 | en_US |
dc.identifier.volume | 54 | en_US |
dc.identifier.issue | 8 | en_US |
dc.identifier.startpage | 6061 | en_US |
dc.identifier.endpage | 6073 | en_US |
dc.relation.ec | info:eu-repo/grantAgreement/TUBITAK/SOBAG/2221 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1007/s12035-016-0137-3 | en_US |
dc.identifier.wosquality | Q1 | en_US |
dc.identifier.scopusquality | Q1 | en_US |