dc.contributor.author | Farshid, Safoura | |
dc.contributor.author | Kharaziha, Mahshid | |
dc.contributor.author | Salehi, Hossein | |
dc.contributor.author | Ganjalikhani Hakemi, Mazdak | |
dc.date.accessioned | 2023-11-13T07:02:53Z | |
dc.date.available | 2023-11-13T07:02:53Z | |
dc.date.issued | 2023 | en_US |
dc.identifier.citation | Farshid, S., Kharaziha, M., Salehi, H. ve Ganjalikhani Hakemi, M. (2023). Morphology-dependent immunomodulatory coating of hydroxyapatite/PEO for magnesium-based bone implants. ACS Applied Materials & Interfaces, 15(42), 48996-49011. https://dx.doi.org/10.1021/acsami.3c11184 | en_US |
dc.identifier.issn | 1944-8244 | |
dc.identifier.issn | 1944-8252 | |
dc.identifier.uri | https://dx.doi.org/10.1021/acsami.3c11184 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/11761 | |
dc.description.abstract | One of the most critical issues concerning orthopedic implants is the risk of chronic inflammation, which poses a threat to the bone healing process. Osteo-immunomodulation plays a pivotal role in implant technology by influencing proinflammatory and anti-inflammatory responses, ultimately promoting bone healing. This study aims to investigate the morphology-dependent osteo-immunomodulatory properties of a hydroxyapatite (HA)/plasma electrolytic oxidation (PEO)-coated WE43 alloy. In this context, following the PEO process with various operational parameters (duty cycles of 50-40, 50-20, 70-40%, and frequencies of 0.5, 0.8, and 1 kHz), a layer of HA was applied as the top coating using a straightforward hot-dip process. The results revealed the formation of the PEO layer with distinct morphologies and pore sizes, depending on the operational parameters. Specifically, a uniform PEO coating with small pore sizes (5.2-5.3 μm) led to the creation of plate-like HA particles, while a random-like HA structure formed on nonuniform surfaces with large pores (7.0-11.1 μm) of PEO. Moreover, it was observed that the plate-like HA coating exhibited higher adhesion strength than the random one (classified as class 2 vs class 3 based on cross-cut standards). Furthermore, electrochemical impedance spectroscopy (EIS) and polarization studies confirmed a substantial increase in the polarization resistance (680 kΩ) and total impedance (48 559.6 Ω) for the plate-like HA/PEO as compared to the substrate (an increase of 1511-fold and 311-fold, respectively) and the random HA/PEO samples (an increase of 85-fold and 18-fold, respectively). In addition, compared to random HA coatings, there was a significant enhancement in the viability (150% control vs 96% control), proliferation, and differentiation of MG63 cells when exposed to plate-like HA coatings. Moreover, surface morphology and chemistry pronouncedly impacted macrophages' viability, morphology, and phenotype. Notably, plate-like HA coatings resulted in a higher upregulation of BMP-2 and TGF-β than proinflammatory cytokines (IL-6 and M-CSF), indicating a polarization of macrophage type 1 (M1) toward type 2 (M2). In summary, the bilayer HA/PEO coating exhibited remarkable osteo-immunomodulatory activity, making it highly appealing for use in bone implant applications. | en_US |
dc.language.iso | eng | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Hydroxyapatite | en_US |
dc.subject | Immunomodulation Activity | en_US |
dc.subject | Magnesium Alloy | en_US |
dc.subject | Orthopedic Implant | en_US |
dc.subject | Plate-Like Morphology | en_US |
dc.title | Morphology-dependent immunomodulatory coating of hydroxyapatite/PEO for magnesium-based bone implants | en_US |
dc.type | article | en_US |
dc.relation.ispartof | ACS Applied Materials & Interfaces | en_US |
dc.department | İstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER) | en_US |
dc.department | İstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü | en_US |
dc.identifier.volume | 15 | en_US |
dc.identifier.issue | 42 | en_US |
dc.identifier.startpage | 48996 | en_US |
dc.identifier.endpage | 49011 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1021/acsami.3c11184 | en_US |
dc.institutionauthor | Ganjalikhani Hakemi, Mazdak | |
dc.identifier.wosquality | Q1 | en_US |
dc.identifier.wos | 001090438700001 | en_US |
dc.identifier.scopus | 2-s2.0-85175270021 | en_US |
dc.identifier.pmid | 37831072 | en_US |
dc.identifier.scopusquality | Q1 | en_US |