Effects of methylprednisolone in the treatment of spinal cord injuries by evaluation of microRNA-21: An experimental study
Erişim
info:eu-repo/semantics/closedAccessTarih
2023Yazar
Abdallah, AnasTekin, Abdurrahim
Öztanır, Mustafa Namık
Süsgün, Seda
Yabacı, Ayşegül
Çınar, İrfan
Can, Engin
Tokar, Sadık
Akbaş, Fahri
Seyithanoğlu, Mehmet Hakan
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Abdallah, A., Tekin, A., Öztanır, M. N., Süsgün, S., Yabacı, A., Çınar, İ. ... Seyithanoğlu, M. H. (2023). Effects of methylprednisolone in the treatment of spinal cord injuries by evaluation of microRNA-21: An experimental study. Journal of Neurological Surgery, Part A: Central European Neurosurgery, 84(3), 240-246. https://doi.org/10.1055/s-0042-1743552Özet
Background and Study Aims Spinal cord injury (SCI) is one of the most complicated pathologies that affect active young males. miR-21 primarily regulates several cellular processes. We aimed to elucidate the regulatory role of miR-21 and test methylprednisolone as a disease-modifying agent on experimental SCI tissues. Methods A total of 36 8- to 10-week-old adult female Sprague-Dawley rats weighing 250 to 300 g were used. Animals were randomly divided into six groups. Except for groups 1 and 4, the spinal trauma model was applied to all animal groups using the clipping method. In groups 3 and 6, methylprednisolone was given. For real-time polymerase chain reaction (PCR) investigations, rats in groups 1, 2, and 3 were reoperated on after the first postoperative day, whereas those in groups 4, 5, and 6 were reoperated on after postoperative day 7 and spinal cord samples from the laminectomy area were removed for gene expression analysis. Relative gene expression of miR-21, Gfap , Vim , Stat3 , Faslg , Pten , Bax , Bcl2 , Cox2 , and Il6 were determined with quantitative reverse transcription (qRT) PCR. Results In group 3, the miR-21 expression significantly increased compared with groups 1 and 2. When compared with group 3, a decrease in miR-21 expression was observed in group 6 ( p < 0.05). When compared with group 4, group 6 had lower levels of Gfap , Pten , Stat3 , and Bax ( p < 0.05). Conclusions miR-21 supports the beneficial aspects of the body's healing mechanisms following SCI. In the acute phase, the use of methylprednisolone increases miR-21 expression in the early period of trauma. Methylprednisolone increases some astrogliosis and inflammation biomarkers' levels; however, it did not affect the apoptotic biomarkers.
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Journal of Neurological Surgery, Part A: Central European NeurosurgeryCilt
84Sayı
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