Yazar "Altun, Hamza Yusuf" için listeleme
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Chemogenetic tools and genetic biosensors in redox biology: Probing Hyper7 signals in specific cellular locales
Altun, Hamza Yusuf; Erdoğan, Yusuf Ceyhun; Seçilmiş, Melike; Eroğlu, Emrah (Elsevier Science Inc., 2023)H2O2 is one of the most studied reactive oxygen species (ROS) in redox biology because of its critical role in oxidant-dependent signaling pathways. Conventional approaches apply exogenous H2O2 on cells and tissues, which ... -
A co-culture-based multiparametric imaging technique to dissect local H2O2 signals with targeted HyPer7
Seçilmiş, Melike; Altun, Hamza Yusuf; Pilic, Johannes; Erdoğan, Yusuf Ceyhun; Çokluk, Zeynep; Ata, Büşra Nur; Sevimli, Gülşah; Zaki, Asal Ghaffari; Yiğit, Esra Nur; Öztürk, Gürkan; Malli, Roland; Eroğlu, Emrah (MDPI, 2021)Multispectral live-cell imaging is an informative approach that permits detecting biological processes simultaneously in the spatial and temporal domain by exploiting spectrally distinct biosensors. However, the combination ... -
Complexities of the chemogenetic toolkit: Differential mDAAO activation by D-amino substrates and subcellular targeting
Erdoğan, Yusuf Can; Altun, Hamza Yusuf; Seçilmiş, Melike; Ata, Büşra N.; Sevimli, Gülşah; Çokluk, Zeynep; Zaki, Asal Ghaffari; Sezen, Serap; Akgül Çağlar, Tuba; Sevgen, İlker; Steinhorn, Benjamin; Ai, Huiwang; Öztürk, Gürkan; Belousov, Vsevelod V.; Michel, Thomas; Eroğlu, Emrah (Elsevier Inc., 2021)A common approach to investigate oxidant-regulated intracellular pathways is to add exogenous H2O2 to living cells or tissues. However, the addition of H2O2 to the culture medium of cells or tissues approach does not ... -
Importance of physiological oxygen levels for endothelial redox signaling using Hyper 7
Altun, Hamza Yusuf; Yang, Fan; Mann, Giovanni; Eroğlu, Emrah (Elsevier Science Inc, 2023)Cellular redox status is tightly linked to ambient oxygen levels. Endothelial cells (EC) in vivo are exposed to O2 levels between 3- 13 kPa, yet the majority in vitro studies maintain EC under atmospheric O2 levels (~18 ... -
Visualizing hydrogen peroxide and nitric oxide dynamics in endothelial cells using multispectral imaging under controlled oxygen conditions
Altun, Hamza Yusuf; Seçilmiş, Melike; Yang, Fan; Akgül Çağlar, Tuba; Vatandaşlar, Emre; Toy, Muhammed Fatih; Vilain, Sven; Mann, Giovanni E.; Öztürk, Gürkan; Eroğlu, Emrah (2024)The complex interplay between hydrogen peroxide (H2O2) and nitric oxide (NO) in endothelial cells presents challenges due to technical limitations in simultaneous measurement, hindering the elucidation of their direct ...