dc.contributor.author | Koç, Arzuhan | |
dc.contributor.author | Çağavi, Esra | |
dc.date.accessioned | 2022-08-12T12:59:16Z | |
dc.date.available | 2022-08-12T12:59:16Z | |
dc.date.issued | 2022 | en_US |
dc.identifier.citation | Koç, A. ve Çağavi, E. (2022). Replating protocol for human induced pluripotent stem cell–derived cardiomyocytes. Methods in Molecular Biology içinde (161-170. ss.). Humana Press Inc. http://doi.org/10.1007/7651_2021_450 | en_US |
dc.identifier.issn | 1064-3745 | |
dc.identifier.issn | 1940-6029 | |
dc.identifier.uri | http://doi.org/10.1007/7651_2021_450 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/9652 | |
dc.description.abstract | Human induced pluripotent stem cell–derived cardiomyocytes (hiPSC-CM) create an unlimited cell source for basic and translational cardiac research. Obtaining hiPSC-CM culture as a single-cell, monolayer or three-dimensional clusters for downstream applications can be challenging. Thus, it is critical to develop replating strategies for hiPSC-CMs by evaluating different enzymatic or nonenzymatic reagents for dissociation and seeding on different coating materials. To reseed hiPSC-CMs with high viability and at structures desirable for the downstream applications, here we defined optimized protocols to dissociate hiPSC-CMs by using collagenase A&B, Collagenase II, TrypLE, and EDTA and reseeding on various matrix materials including fibronectin, laminin, imatrix, Matrigel, and Geltrex. By the replating methods described here, a single cell or cluster-containing hiPSC-CM cultures can be generated effectively. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Humana Press Inc. | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Cardiac Differentiation | en_US |
dc.subject | Cardiomyocytes | en_US |
dc.subject | Cell Dissociation | en_US |
dc.subject | Human Induced Pluripotent Stem Cells | en_US |
dc.subject | Replating Cardiomyocytes | en_US |
dc.title | Replating protocol for human induced pluripotent stem cell–derived cardiomyocytes | en_US |
dc.type | bookPart | en_US |
dc.relation.ispartof | Methods in Molecular Biology | en_US |
dc.department | İstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER) | en_US |
dc.department | İstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü | en_US |
dc.department | İstanbul Medipol Üniversitesi, Sağlık Bilimleri Enstitüsü, Mikrobiyoloji Ana Bilim Dalı | en_US |
dc.department | İstanbul Medipol Üniversitesi, Sağlık Bilimleri Enstitüsü, Tıbbi Biyoloji ve Genetik Ana Bilim Dalı | en_US |
dc.department | İstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Ana Bilim Dalı | en_US |
dc.authorid | 0000-0002-4022-1014 | en_US |
dc.authorid | 0000-0002-7199-583X | en_US |
dc.identifier.volume | 2520 | en_US |
dc.identifier.startpage | 161 | en_US |
dc.identifier.endpage | 170 | en_US |
dc.relation.publicationcategory | Kitap Bölümü - Uluslararası | en_US |
dc.identifier.doi | 10.1007/7651_2021_450 | en_US |
dc.institutionauthor | Koç, Arzuhan | |
dc.institutionauthor | Çağavi, Esra | |
dc.identifier.scopus | 2-s2.0-85135282585 | en_US |
dc.identifier.pmid | 34845657 | en_US |
dc.identifier.scopusquality | Q4 | en_US |