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dc.contributor.authorKoç, Arzuhan
dc.contributor.authorÇağavi, Esra
dc.date.accessioned2022-08-12T12:59:16Z
dc.date.available2022-08-12T12:59:16Z
dc.date.issued2022en_US
dc.identifier.citationKoç, A. ve Çağavi, E. (2022). Replating protocol for human induced pluripotent stem cell–derived cardiomyocytes. Methods in Molecular Biology içinde (161-170. ss.). Humana Press Inc. http://doi.org/10.1007/7651_2021_450en_US
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.urihttp://doi.org/10.1007/7651_2021_450
dc.identifier.urihttps://hdl.handle.net/20.500.12511/9652
dc.description.abstractHuman induced pluripotent stem cell–derived cardiomyocytes (hiPSC-CM) create an unlimited cell source for basic and translational cardiac research. Obtaining hiPSC-CM culture as a single-cell, monolayer or three-dimensional clusters for downstream applications can be challenging. Thus, it is critical to develop replating strategies for hiPSC-CMs by evaluating different enzymatic or nonenzymatic reagents for dissociation and seeding on different coating materials. To reseed hiPSC-CMs with high viability and at structures desirable for the downstream applications, here we defined optimized protocols to dissociate hiPSC-CMs by using collagenase A&B, Collagenase II, TrypLE, and EDTA and reseeding on various matrix materials including fibronectin, laminin, imatrix, Matrigel, and Geltrex. By the replating methods described here, a single cell or cluster-containing hiPSC-CM cultures can be generated effectively.en_US
dc.language.isoengen_US
dc.publisherHumana Press Inc.en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCardiac Differentiationen_US
dc.subjectCardiomyocytesen_US
dc.subjectCell Dissociationen_US
dc.subjectHuman Induced Pluripotent Stem Cellsen_US
dc.subjectReplating Cardiomyocytesen_US
dc.titleReplating protocol for human induced pluripotent stem cell–derived cardiomyocytesen_US
dc.typebookParten_US
dc.relation.ispartofMethods in Molecular Biologyen_US
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)en_US
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsüen_US
dc.departmentİstanbul Medipol Üniversitesi, Sağlık Bilimleri Enstitüsü, Mikrobiyoloji Ana Bilim Dalıen_US
dc.departmentİstanbul Medipol Üniversitesi, Sağlık Bilimleri Enstitüsü, Tıbbi Biyoloji ve Genetik Ana Bilim Dalıen_US
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Ana Bilim Dalıen_US
dc.authorid0000-0002-4022-1014en_US
dc.authorid0000-0002-7199-583Xen_US
dc.identifier.volume2520en_US
dc.identifier.startpage161en_US
dc.identifier.endpage170en_US
dc.relation.publicationcategoryKitap Bölümü - Uluslararasıen_US
dc.identifier.doi10.1007/7651_2021_450en_US
dc.institutionauthorKoç, Arzuhan
dc.institutionauthorÇağavi, Esra
dc.identifier.scopus2-s2.0-85135282585en_US
dc.identifier.pmid34845657en_US
dc.identifier.scopusqualityQ4en_US


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