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dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorAkalan, Hande
dc.contributor.authorÖznam, Kadir
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorYaşar Şirin, Duygu
dc.contributor.authorÖzbek, Hanefi
dc.date.accessioned2022-07-29T11:50:27Z
dc.date.available2022-07-29T11:50:27Z
dc.date.issued2022en_US
dc.identifier.citationYılmaz, İ., Akalan, H., Öznam, K., Karaarslan, N., Yaşar Şirin, D. ve Özbek, H. (2022). Does oseltamivir protect human chondrocyte and nucleus pulposus cells from degeneration by inhibiting senescence and proinflammation mediated by the NLRP3 inflammasome and NF-κB? European Review for Medical and Pharmacological Sciences, 26(13), 4816-4827. https://doi.org/10.26355/eurrev_202207_29207en_US
dc.identifier.issn1128-3602
dc.identifier.urihttps://doi.org/10.26355/eurrev_202207_29207
dc.identifier.urihttps://hdl.handle.net/20.500.12511/9625
dc.description.abstractOBJECTIVE: Recent drug design studies suggest that inflammation is among the most important factors in the development of both intervertebral disc (IVD) degeneration (IVDD) and osteoarthritis (OA) due to cartilage damage. This study aimed to investigate whether the anti-inflammatory drug oseltamivir has a toxic effect on IVD and cartilage tissue cells. It assessed what effect oseltamivir has on hypoxia-inducible factor (HIF)-1 alpha (HIF1α), which plays an important role in anabolic pathways in IVD and cartilage tissue. In addition, the study analyzed whether oseltamivir could inhibit the release of inflammatory interleukin-1 beta (IL-1β) via the nuclear factor kappa-B (NF-κB) signaling pathway by activating the nucleotide-binding oligomerization domain and leucine-rich repeat protein-3 (NLRP3) inflammasome. MATERIALS AND METHODS: Human lumbar IVD (n = 8) tissues were isolated for annulus fibrosus (AF) and nucleus pulposus (NP) primary cell cultures, and human tibial and femoral cartilage tissues (n = 8) were isolated for primary chondrocyte cultures. Untreated groups served as the control and oseltamivir-treated groups as the study sample. Cell viability and cytotoxicity were evaluated at 0, 24, 48, and 72 h in all groups for changes in HIF-1α, IL-1β, NF-κB, and the NLRP3-inflammasome protein expressions using Western blotting. The α significance value was < 0.05. RESULTS: In the oseltamivir-treated groups, cell proliferation decreased in both AF/NP cell and chondrocyte cultures obtained from IVD cartilage tissues. After Western blotting analysis, changes were observed in the protein expressions of HIF-1α, IL-1β, NF-κB, and the NLRP3 inflammasome in both AF/NP cells and chondrocytes. The results were statistically significant (p < 0.05). CONCLUSIONS: Oseltamivir treatment may be a promising regenerative strategy to manage IVDD and osteoarthritic cartilage tissues.en_US
dc.language.isoengen_US
dc.publisherVerduci Editore s.r.len_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectDegenerationen_US
dc.subjectInflammationen_US
dc.subjectNF-κBen_US
dc.subjectNLRP3 Inflammasomeen_US
dc.subjectOseltamiviren_US
dc.titleDoes oseltamivir protect human chondrocyte and nucleus pulposus cells from degeneration by inhibiting senescence and proinflammation mediated by the NLRP3 inflammasome and NF-κB?en_US
dc.title.alternativeDoes oseltamivir protect human chondrocyte and nucleus pulposus cells from degeneration by inhibiting senescence and proinflammation mediated by the NLRP3 inflammasome and NF-kappa B?en_US
dc.title.alternativeDoes oseltamivir protect human chondrocyte and nucleus pulposus cells from degeneration by inhibiting senescence and proinflammation mediated by the NLRP3 inflammasome and NF-kappa B?en_US
dc.typearticleen_US
dc.relation.ispartofEuropean Review for Medical and Pharmacological Sciencesen_US
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Ortopedi ve Travmatoloji Ana Bilim Dalıen_US
dc.authorid0000-0001-7392-7729en_US
dc.authoridQ3en_US
dc.identifier.volume26en_US
dc.identifier.issue13en_US
dc.identifier.startpage4816en_US
dc.identifier.endpage4827en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.26355/eurrev_202207_29207en_US
dc.institutionauthorÖznam, Kadir
dc.identifier.wosqualityQ2en_US
dc.identifier.wos000837256500034en_US
dc.identifier.scopus2-s2.0-85134249183en_US
dc.identifier.pmid35856374en_US
dc.identifier.scopusqualityQ2en_US


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