Evaluation of apoptosis pathway of geraniol on ishikawa cells
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info:eu-repo/semantics/embargoedAccessTarih
2021Yazar
Kuzu, BetülCüce, Gökhan
Çınar Ayan, İlknur
Gültekin, Burcu
Canbaz, Halime Tuba
Dursun, Hatice Gül
Şahin, Zafer
Keskin, İlknur
Kalkan, Sabiha Serpil
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Kuzu, B., Cüce, G., Çınar Ayan, İ., Gültekin, B., Canbaz, H. T., Dursun, H. G. ... Kalkan, S. S. (2021). Evaluation of apoptosis pathway of geraniol on ishikawa cells. Nutrition and Cancer, 73(11-12), 2532-2537. https://doi.org/10.1080/01635581.2020.1836244Özet
Endometrial cancer is the most common type of cancer in the female reproductive system. Geraniol is acyclic monoterpene alcohol derived from essential oils of aromatic plants. This study aimed to investigate the apoptosis pathway of geraniol on Ishikawa cells. The cytotoxic effects of Geraniol on Ishikawa cells were determined by an MTT test. Ishikawa cells were seeded on cover slips, the IC50 dose was applied, and the cells were incubated with antibodies against Bax, Bcl-2, and TUNEL Assay. mRNA expression analysis of apoptosis-related genes was determined by RT-qPCR with an IC50 dose of Geraniol. The IC50 dose of Geraniol decreased Bcl-2 staining significantly, but it significantly increased Bax staining and TUNEL positive cells. A significant increase in the Bax, caspase3, caspase-8, cytochrome C and Fas genes and a significant decrease in the Bcl-2 gene was observed when the IC50 dose group was compared to the cells in the control group based on their mRNA expression levels.Analysis of expression of genes whose products are involved in apoptosis suggests the involvement of the mitochondrial pathway.
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Nutrition and CancerCilt
73Sayı
11-12Koleksiyonlar
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