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dc.contributor.authorJanssen, Lisa
dc.contributor.authorAi, Xiaoyu
dc.contributor.authorZheng, Xuan
dc.contributor.authorWei, Wei
dc.contributor.authorÇağlayan, Ahmet Burak
dc.contributor.authorKılıç, Ertuğrul
dc.contributor.authorWang, Ya-chao
dc.contributor.authorHermann, Dirk M.
dc.contributor.authorVenkataramani, Vivek
dc.contributor.authorBaehr, Mathias
dc.contributor.authorDoeppner, Thorsten R.
dc.date.accessioned2021-09-16T08:34:50Z
dc.date.available2021-09-16T08:34:50Z
dc.date.issued2021en_US
dc.identifier.citationJanssen, L., Ai, X., Zheng, X., Wei, W., Çağlayan, A. B., Kılıç, E. ... Doeppner, T. R. (2021). Inhibition of fatty acid synthesis aggravates brain injury, reduces blood-brain barrier integrity and impairs neurological recovery in a murine stroke model. Frontiers in Cellular Neuroscience, 15. https://dx.doi.org/10.3389/fncel.2021.733973en_US
dc.identifier.issn1662-5102
dc.identifier.urihttps://dx.doi.org/10.3389/fncel.2021.733973
dc.identifier.urihttps://hdl.handle.net/20.500.12511/8191
dc.description.abstractInhibition of fatty acid synthesis (FAS) stimulates tumor cell death and reduces angiogenesis. When SH-SY5Y cells or primary neurons are exposed to hypoxia only, inhibition of FAS yields significantly enhanced cell injury. The pathophysiology of stroke, however, is not only restricted to hypoxia but also includes reoxygenation injury. Hence, an oxygen-glucose-deprivation (OGD) model with subsequent reoxygenation in both SH-SY5Y cells and primary neurons as well as a murine stroke model were used herein in order to study the role of FAS inhibition and its underlying mechanisms. SH-SY5Y cells and cortical neurons exposed to 10 h of OGD and 24 h of reoxygenation displayed prominent cell death when treated with the Acetyl-CoA carboxylase inhibitor TOFA or the fatty acid synthase inhibitor cerulenin. Such FAS inhibition reduced the reduction potential of these cells, as indicated by increased NADH(2)(+)/NAD(+) ratios under both in vitro and in vivo stroke conditions. As observed in the OGD model, FAS inhibition also resulted in increased cell death in the stroke model. Stroke mice treated with cerulenin did not only display increased brain injury but also showed reduced neurological recovery during the observation period of 4 weeks. Interestingly, cerulenin treatment enhanced endothelial cell leakage, reduced transcellular electrical resistance (TER) of the endothelium and contributed to poststroke blood-brain barrier (BBB) breakdown. The latter was a consequence of the activated NF-kappa B pathway, stimulating MMP-9 and ABCB1 transporter activity on the luminal side of the endothelium. In conclusion, FAS inhibition aggravated poststroke brain injury as consequence of BBB breakdown and NF-kappa B-dependent inflammation.en_US
dc.language.isoengen_US
dc.publisherFrontiers Media SAen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectBlood-Brain Barrieren_US
dc.subjectCerebral Ischemiaen_US
dc.subjectFatty Acid Synthesisen_US
dc.subjectHypoxiaen_US
dc.subjectNeuroprotectionen_US
dc.subjectReduction Potentialen_US
dc.titleInhibition of fatty acid synthesis aggravates brain injury, reduces blood-brain barrier integrity and impairs neurological recovery in a murine stroke modelen_US
dc.typearticleen_US
dc.relation.journalFrontiers in Cellular Neuroscienceen_US
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)en_US
dc.authorid0000-0002-6242-3709en_US
dc.authorid0000-0001-6494-8923en_US
dc.identifier.volume15en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.3389/fncel.2021.733973en_US


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