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dc.contributor.authorAyla, Şule
dc.contributor.authorTunalı, Gülden
dc.contributor.authorBilgiç, Bülent Emre
dc.contributor.authorSofuoğlu, Kenan
dc.contributor.authorÖzdemir, A. Arman
dc.contributor.authorTanrıverdi, Gamze
dc.contributor.authorÖzdemir, Semra
dc.contributor.authorSoner, Burak Cem
dc.contributor.authorÖztürk, Bahar
dc.contributor.authorKarahüseyinoğlu, Serçin
dc.contributor.authorAslan, Esra
dc.contributor.authorSeçkin, İsmail
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:35:27Z
dc.date.available10.07.201910:49:14
dc.date.available2019-07-10T19:35:27Z
dc.date.issued2018en_US
dc.identifier.citationAyla, Ş., Tunalı, G., Bilgiç, B., Sofuoğlu, K., Özdemir, A., Tanrıverdi, G. ... Seçkin, İ. (2018). Antioxidant activity of CAPE (Caffeic Acid Phenethyl Ester) in vitro can protect human sperm deoxyribonucleic acid from oxidative damage. Acta Histochemica, 120(2), 117-121. https://dx.doi.org/10.1016/j.acthis.2018.01.001en_US
dc.identifier.issn0065-1281
dc.identifier.issn1618-0372
dc.identifier.urihttps://hdl.handle.net/20.500.12511/787
dc.identifier.urihttps://dx.doi.org/10.1016/j.acthis.2018.01.001
dc.description.abstractPurpose: Sperm processing (e.g., centrifugation) used in preparation for assisted reproduction can result in excessive generation of reactive oxygen species (ROS) and potential sperm damage. The use of antioxidants during sperm processing has been shown to prevent iatrogenic sperm damage, including DNA damage. In this study, we evaluated the effect of caffeic acid phenethyl ester (CAPE) on oxidative stress mediated sperm dysfunction and DNA damage. Methods: Semen samples were obtained to liquefy at room temperature. After centrifugation and washing protocols, spermatozoa were incubated in a single step supplemented medium with either of 10, 50 or 100 µmol/L CAPE for 2 hours at 36 °C. After incubation period, MDA levels of seminal plasma were measured. The fragmentation in sperm DNA was detected by light microscopy via use of an aniline blue assay, while ultrastructural morphology was analyzed by transmission electron microscopy. Results: Significant increase has been observed in percent chromatin condensation (assessed by aniline blue staining) and Malondialdehyde (Mmol/L) in oligoasthenoteratozoospermia group before the centrifugation (0.57 ± 0.15). Incubation of samples with 100 µmol/L CAPE after centrifugation resulted in a significantly lower percent chromatin condensation compared to samples incubated without CAPE (0.42 ± 0.12) (P <0.0033). Incubation of all samples with CAPE (10 µmol/L, 50 µmol/L, 100 µmol/L.) after centrifugation resulted in a significantly lower percentage of Malondialdehyde levels. Conclusions: The data suggests that preincubation of spermatozoa with the antioxidant CAPE offers protection against oxidative DNA damage in vitro.en_US
dc.description.sponsorshipThe Ministry of Economic Affairs and Employmenten_US
dc.language.isoengen_US
dc.publisherElsevier GmbHen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAntioxidanten_US
dc.subjectCAPEen_US
dc.subjectSperm DNA Damageen_US
dc.subjectSperm Motilityen_US
dc.titleAntioxidant activity of CAPE (Caffeic Acid Phenethyl Ester) in vitro can protect human sperm deoxyribonucleic acid from oxidative damageen_US
dc.typearticleen_US
dc.relation.journalActa Histochemicaen_US
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalıen_US
dc.authorid0000-0003-2143-5268en_US
dc.identifier.volume120en_US
dc.identifier.issue2en_US
dc.identifier.startpage117en_US
dc.identifier.endpage121en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1016/j.acthis.2018.01.001en_US


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