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dc.contributor.authorKoç, Arzuhan
dc.contributor.authorŞahoğlu Göktaş, Sevilay
dc.contributor.authorAkgül Çağlar, Tuba
dc.contributor.authorÇağavi, Esra
dc.date.accessioned2021-05-21T08:09:52Z
dc.date.available2021-05-21T08:09:52Z
dc.date.issued2021en_US
dc.identifier.citationKoç, A., Şahoğlu Göktaş, S., Akgül Çağlar, T. ve Çağavi, E. (2021). Defining optimal enzyme and matrix combination for replating of human induced pluripotent stem cell-derived cardiomyocytes at different levels of maturity. Experimental Cell Research, 403(2). https://dx.doi.org/10.1016/j.yexcr.2021.112599en_US
dc.identifier.issn0014-4827
dc.identifier.issn1090-2422
dc.identifier.urihttps://dx.doi.org/10.1016/j.yexcr.2021.112599
dc.identifier.urihttps://hdl.handle.net/20.500.12511/6874
dc.description.abstractHuman induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) create an unlimited cell source for basic and translational research. Depending on the maturity of cardiac cultures and the intended applications, obtaining hiPSC-CMs as a single-cell, monolayer or three-dimensional clusters can be challenging. Here, we defined strategies to replate hiPSC-CMs on early days (D15-30) or later more mature (D60-150) differentiation cultures. After generation of hiPSCs and derivation of cardiomyocytes, four dissociation reagents Collagenase A/B, Collagenase II, TrypLE, EDTA and five different extracellular matrix materials Laminin, iMatrix-511, Fibronectin, Matrigel, and Geltrex were comparatively evaluated by imaging, cell viability, and contraction analysis. For early cardiac differentiation cultures mimicking mostly the embryonic stage, the highest adhesion, cell viability, and beating frequencies were achieved by treatment with the TrypLE enzyme. Video-based contraction analysis demonstrated higher beating rates after replating compared to before treatment. For later differentiation days of more mature cardiac cultures, dissociation with EDTA and replating cells on Geltrex or Laminin-derivatives yielded better recovery. Cardiac clusters at various sizes were detected in several groups treated with collagenases. Collectively, our findings revealed the selection criteria of the dissociation approach and coating matrix for replating iPSC-CMs based on the maturity and the requirements of further downstream applications.en_US
dc.language.isoengen_US
dc.publisherElsevier Inc.en_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectHuman Induced Pluripotent Stem Cellsen_US
dc.subjectCardiomyocytesen_US
dc.subjectCardiac Differentiationen_US
dc.subjectReplatingen_US
dc.subjectCell Dissociationen_US
dc.subjectDissociation Enzymesen_US
dc.subjectExtracellular Matrixen_US
dc.titleDefining optimal enzyme and matrix combination for replating of human induced pluripotent stem cell-derived cardiomyocytes at different levels of maturityen_US
dc.typearticleen_US
dc.relation.journalExperimental Cell Researchen_US
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)en_US
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsüen_US
dc.departmentİstanbul Medipol Üniversitesi, Sağlık Bilimleri Enstitüsü, Mikrobiyoloji Ana Bilim Dalıen_US
dc.departmentİstanbul Medipol Üniversitesi, Sağlık Bilimleri Enstitüsü, Sinirbilim Ana Bilim Dalıen_US
dc.departmentİstanbul Medipol Üniversitesi, Sağlık Bilimleri Enstitüsü, Tıbbi Biyoloji ve Genetik Ana Bilim Dalıen_US
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Ana Bilim Dalıen_US
dc.authorid0000-0002-4022-1014en_US
dc.authorid0000-0002-7199-583Xen_US
dc.identifier.volume403en_US
dc.identifier.issue2en_US
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/119S132
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/213S192
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1016/j.yexcr.2021.112599en_US


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