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dc.contributor.authorBayat, Fazlı Kemal
dc.contributor.authorPolat Budak, Betül
dc.contributor.authorYiğit, Esra Nur
dc.contributor.authorÖztürk, Gürkan
dc.contributor.authorGülçür, Halil Özcan
dc.contributor.authorGüveniş, Albert
dc.date.accessioned2021-03-26T08:12:37Z
dc.date.available2021-03-26T08:12:37Z
dc.date.issued2021en_US
dc.identifier.citationBayat, F. K., Polat Budak, B., Yiğit, E. N., Öztürk, G., Gülçür, H. Ö. ve Güveniş, A. (2021). Adult mouse dorsal root ganglia neurons form aberrant glutamatergic connections in dissociated cultures. PLoS One, 16(3). https://dx.doi.org/10.1371/journal.pone.0246924en_US
dc.identifier.issn1932-6203
dc.identifier.urihttps://dx.doi.org/10.1371/journal.pone.0246924
dc.identifier.urihttps://hdl.handle.net/20.500.12511/6657
dc.description.abstractCultured sensory neurons can exhibit complex activity patterns following stimulation in terms of increased excitability and interconnected responses of multiple neurons. Although these complex activity patterns suggest a network-like configuration, research so far had little interest in synaptic network formation ability of the sensory neurons. To identify interaction profiles of Dorsal Root Ganglia (DRG) neurons and explore their putative connectivity, we developed an in vitro experimental approach. A double transgenic mouse model, expressing genetically encoded calcium indicator (GECI) in their glutamatergic neurons, was produced. Dissociated DRG cultures from adult mice were prepared with a serum-free protocol and no additional growth factors or cytokines were utilized for neuronal sensitization. DRG neurons were grown on microelectrode arrays (MEA) to induce stimulus-evoked activity with a modality-free stimulation strategy. With an almost single-cell level electrical stimulation, spontaneous and evoked activity of GCaMP6s expressing neurons were detected under confocal microscope. Typical responses were analyzed, and correlated calcium events were detected across individual DRG neurons. Next, correlated responses were successfully blocked by glutamatergic receptor antagonists, which indicated functional synaptic coupling. Immunostaining confirmed the presence of synapses mainly in the axonal terminals, axon-soma junctions and axon-axon intersection sites. Concisely, the results presented here illustrate a new type of neuron-to-neuron interaction in cultured DRG neurons conducted through synapses. The developed assay can be a valuable tool to analyze individual and collective responses of the cultured sensory neurons.en_US
dc.description.sponsorshipThis work is funded by Bogazici University Research Fund to author AG under the Project Code 8080D. During the experiments some facilities of REMER (Istanbul Medipol University, Regenerative and Restorative Medicine Center) were used. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en_US
dc.description.sponsorshipBogazici Universityen_US
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectDissociated Culturesen_US
dc.subjectGlutamatergic Connectionsen_US
dc.subjectRoot Ganglia Neuronsen_US
dc.titleAdult mouse dorsal root ganglia neurons form aberrant glutamatergic connections in dissociated culturesen_US
dc.typearticleen_US
dc.relation.journalPLoS Oneen_US
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)en_US
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsüen_US
dc.authorid0000-0001-9037-5217en_US
dc.identifier.volume16en_US
dc.identifier.issue3en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1371/journal.pone.0246924en_US


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