Methylnitrosourea, dimethylbenzanthracene and benzoapyrene differentially affect redox pathways, apoptosis and immunity in zebrafish
Üstündağ, Ünsal Veli
Ateş, Perihan Seda
Üstündağ, F. D.
Alturfan, Ahmet Ata
Adil Altınöz, Meriç
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KünyeEğimezer, G., Üstündağ, Ü. V., Ateş, P. S., Ünal, İ., Üstündağ, F. D., Alturfan, A. A. ... Elmacı, İ. (2020). Methylnitrosourea, dimethylbenzanthracene and benzoapyrene differentially affect redox pathways, apoptosis and immunity in zebrafish. Human and Experimental Toxicology, 39(7), 920-929. https://dx.doi.org/10.1177/0960327120905961
Cancer continues to be a major cause of mortality globally. Zebrafish present suitable models for studying the mechanisms of genotoxic carcinogens. The aim of this study was to investigate the interaction between oxidant-antioxidant status, apoptosis and immunity in zebrafish that were exposed to three different genotoxic carcinogens methylnitrosourea, dimethylbenzanthracene, benzoapyrene and methylnitrosourea + dimethylbenzanthracene starting from early embryogenesis for 30 days. Lipid peroxidation, nitric oxide levels, superoxide dismutase and glutathione-S-transferase activities and mRNA levels of apoptosis genes p53, bax, casp3a, casp2 and immunity genes fas, tnf alpha and ifn gamma 1 were evaluated. The disruption of the oxidant-antioxidant balance accompanied by altered expressions of apoptotic and immunity related genes were observed in different levels according to the carcinogen applied. Noteworthy, ifn gamma expressions decreased in all carcinogen-exposed groups. Our results will provide basic data for further carcinogenesis research in zebrafish models.