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dc.contributor.authorVerimli, Nihan
dc.contributor.authorDemiral, Ayşegül
dc.contributor.authorYılmaz, Hülya
dc.contributor.authorÇulha, Mustafa
dc.contributor.authorErdem, Sultan Sibel
dc.date.accessioned2020-01-29T06:17:05Z
dc.date.available2020-01-29T06:17:05Z
dc.date.issued2019en_US
dc.identifier.citationVerimli, N., Demiral, A., Yılmaz, A., Çulha, M. ve Erdem, S. S. (2019). Design of dense brush conformation bearing gold nanoparticles as theranostic agent for cancer. Applied Biochemistry and Biotechnology, 189(3), 709-728. https://doi.org/10.1007/s12010-019-03151-6en_US
dc.identifier.issn0273-2289
dc.identifier.issn1559-0291
dc.identifier.urihttps://doi.org/10.1007/s12010-019-03151-6.
dc.identifier.urihttps://hdl.handle.net/20.500.12511/4932
dc.description.abstractDense brush conformation–bearing theranostic agents are emerging as drug delivery systems due to their higher ability to escape from reticuloendothelial system uptake which prolongs their in vivo circulation time. With the aim of developing dual therapy agent, 13-nm gold nanoparticles’ (AuNPs) surfaces were coated with different amounts of polyethylene glycol (PEG) (SH-PEG-NH2) to obtain dense brush conformation–bearing theranostic agents. Among the 14 different theranostic agent candidates prepared, the one hosting 1819 PEG per particle was selected as the most promising theranostic agent candidate based on structural conformation, stability, size, zeta potential, hemocompatibility, cell inhibition, and cell death pathway towards MCF-7 cell line. To test drug delivery efficiency of the developed PEGylated AuNP and to improve efficacy of the treatment, apoptotic peptide (AP) was covalently conjugated to NH2 terminus of the PEG in various ratios to yield AuNP-AP conjugate. Among the prepared conjugates, the one having 1 nmol of peptide per milliliter of AuNP yielded the most promising results based on the same criteria as employed for PEGylated AuNPs. Besides, incorporation of AP to AuNP returned in superior efficacy of AP since it was possible to achieve 50% cell death with 1000 times less amount of AP alone.en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectApoptosisen_US
dc.subjectApoptotic Peptideen_US
dc.subjectBreast Canceren_US
dc.subjectDrug Deliveryen_US
dc.subjectGold Nanoparticlesen_US
dc.subjectHemocompatibilityen_US
dc.subjectPolyethylene Glycolen_US
dc.subjectTheranostic Agenten_US
dc.titleDesign of dense brush conformation bearing gold nanoparticles as theranostic agent for canceren_US
dc.typearticleen_US
dc.relation.ispartofApplied Biochemistry and Biotechnologyen_US
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalıen_US
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)en_US
dc.departmentİstanbul Medipol Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümüen_US
dc.identifier.volume189en_US
dc.identifier.issue3en_US
dc.identifier.startpage709en_US
dc.identifier.endpage728en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1007/s12010-019-03151-6en_US
dc.identifier.wosqualityQ3en_US
dc.identifier.scopusqualityQ2en_US


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