dc.contributor.author | Atasoy, Deniz | |
dc.contributor.author | Betley, Jan Nicholas | |
dc.contributor.author | Li, Wei-Ping | |
dc.contributor.author | Hong Su, Helen | |
dc.contributor.author | Sertel, Sinem M. | |
dc.contributor.author | Scheffer, Louis K. | |
dc.contributor.author | Simpson, Julie H. | |
dc.contributor.author | Fetter, Richard D. | |
dc.contributor.author | Sternson, Scott M. | |
dc.date.accessioned | 10.07.201910:49:13 | |
dc.date.accessioned | 2019-07-10T20:01:53Z | |
dc.date.available | 10.07.201910:49:13 | |
dc.date.available | 2019-07-10T20:01:53Z | |
dc.date.issued | 2014 | en_US |
dc.identifier.citation | Atasoy, D., Betley, J. N., Li, W. P., Hong Su, H., Sertel, S. M., Scheffer, L. K. ... Sternson, S. M. (2014). A genetically specified connectomics approach applied to long-range feeding regulatory circuits. Nature Neuroscience, 17(12), 1830-1839. https://dx.doi.org/10.1038/nn.3854 | en_US |
dc.identifier.issn | 1097-6256 | |
dc.identifier.issn | 1546-1726 | |
dc.identifier.uri | https://dx.doi.org/10.1038/nn.3854 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/3480 | |
dc.description | WOS: 000345484000033 | en_US |
dc.description | PubMed ID: 25362474 | en_US |
dc.description.abstract | Synaptic connectivity and molecular composition provide a blueprint for information processing in neural circuits. Detailed structural analysis of neural circuits requires nanometer resolution, which can be obtained with serial-section electron microscopy. However, this technique remains challenging for reconstructing molecularly defined synapses. We used a genetically encoded synaptic marker for electron microscopy (GESEM) based on intra-vesicular generation of electron-dense labeling in axonal boutons. This approach allowed the identification of synapses from Cre recombinase expressing or GAL4-expressing neurons in the mouse and fly with excellent preservation of ultrastructure. We applied this tool to visualize long-range connectivity of AGRP and POMC neurons in the mouse, two molecularly defined hypothalamic populations that are important for feeding behavior. Combining selective ultrastructural reconstruction of neuropil with functional and viral circuit mapping, we characterized some basic features of circuit organization for axon projections of these cell types. Our findings demonstrate that GESEM labeling enables long-range connectomics with molecularly defined cell types. | en_US |
dc.description.sponsorship | Howard Hughes Medical Institute | en_US |
dc.description.sponsorship | We thank A. Wardlaw for mouse breeding and genotyping, K. Morris for HSV stereotaxic injections, A. Hu and M. Copeland for histology, and L. Lo, D. Anderson and R. Gong with advice on HSV129 anterograde tracing. This research was funded by the Howard Hughes Medical Institute. The HSV129 Delta TK-TT anterograde trans-synaptic tracer virus was provided by the Center for Neuroanatomy with Neurotropic Viruses (P40RR018604). | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Genetically Specified | en_US |
dc.subject | Regulatory Circuits | en_US |
dc.title | A genetically specified connectomics approach applied to long-range feeding regulatory circuits | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Nature Neuroscience | en_US |
dc.department | İstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı | en_US |
dc.department | İstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER) | en_US |
dc.authorid | 0000-0002-3325-8820 | en_US |
dc.identifier.volume | 17 | en_US |
dc.identifier.issue | 12 | en_US |
dc.identifier.startpage | 1830 | en_US |
dc.identifier.endpage | 1839 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1038/nn.3854 | en_US |
dc.identifier.wosquality | Q1 | en_US |
dc.identifier.scopusquality | Q1 | en_US |