Calculation of the Ki67 index in pancreatic neuroendocrine tumors: A comparative analysis of four counting methodologies
Göster/ Aç
Erişim
info:eu-repo/semantics/openAccessTarih
2015Yazar
Reid, Michelle DianBağcı, Pelin
Ohike, Nobuyuki
Saka, Burcu
Erbarut Seven, İpek
Dursun, Nevra
Balcı, Serdar
Gucer, Hasan
Jang, Kee-Taek
Tajiri, Takuma
Baştürk, Olca
Kong, So Yeon
Goodman, Michael
Akkaş, Gizem
Adsay, Volkan
Üst veri
Tüm öğe kaydını gösterKünye
Reid, M., Bağcı, P., Ohike, N., Saka, B., Erbarut Seven, İ., Dursun, N. ... Adsay, V. (2015). Calculation of the Ki67 index in pancreatic neuroendocrine tumors: A comparative analysis of four counting methodologies. Modern Pathology, 28(5), 686-694. https://dx.doi.org/10.1038/modpathol.2014.156Özet
Ki67 index is now an essential part of classification of pancreatic neuroendocrine tumors. However, its adaptation into daily practice has been fraught with challenges related to counting methodology. In this study, three reviewers used four counting methodologies to calculate Ki67 index in 68 well-differentiated pancreatic neuroendocrine tumors: (1) 'eye-ball' estimation, which has been advocated as reliable and is widely used; (2) automated counting by image analyzer; (3) manual eye-counting (eye under a microscope without a grid); and (4) manual count of camera-captured/printed image. Pearson's correlation (R) was used to measure pair-wise correlation among three reviewers using all four methodologies. Average level of agreement was calculated using mean of R values. The results showed that: (1) 'eye-balling' was least expensive and fastest (average time <1 min) but had poor reliability and reproducibility. (2) Automated count was the most expensive and least practical with major impact on turnaround time (limited by machine and personnel accessibility), and, more importantly, had inaccuracies in overcounting unwanted material. (3) Manual eye count had no additional cost, averaged 6 min, but proved impractical and poorly reproducible. (4) Camera-captured/printed image was most reliable, had highest reproducibility, but took longer than 'eye-balling'. In conclusion, based on its comparatively low cost/benefit ratio and reproducibility, camera-captured/printed image appears to be the most practical for calculating Ki67 index. Although automated counting is generally advertised as the gold standard for index calculation, in this study it was not as accurate or cost-effective as camera-captured/printed image and was highly operator-dependent. 'Eye-balling' produces highly inaccurate and unreliable results, and is not recommended for routine use.
WoS Q Kategorisi
Q1Scopus Q Kategorisi
Q1Kaynak
Modern PathologyCilt
28Sayı
5Koleksiyonlar
- Makale Koleksiyonu [3651]
- PubMed İndeksli Yayınlar Koleksiyonu [4049]
- Scopus İndeksli Yayınlar Koleksiyonu [6285]
- WoS İndeksli Yayınlar Koleksiyonu [6432]