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dc.contributor.authorSozen, Berna
dc.contributor.authorÖztürk, Saffet
dc.contributor.authorYaba, Aylin
dc.contributor.authorDemir, Necdet
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T20:01:41Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T20:01:41Z
dc.date.issued2015en_US
dc.identifier.citationSozen, B., Öztürk, S., Yaba, A. ve Demir, N. (2015). The p38 MAPK signalling pathway is required for glucose metabolism, lineage specification and embryo survival during mouse preimplantation development. Mechanisms of Development, 138, 375-398. https://dx.doi.org/10.1016/j.mod.2015.05.002en_US
dc.identifier.issn0925-4773
dc.identifier.issn1872-6356
dc.identifier.urihttps://dx.doi.org/10.1016/j.mod.2015.05.002
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3404
dc.descriptionWOS: 000365991800016en_US
dc.descriptionPubMed ID: 26025760en_US
dc.description.abstractPreimplantation embryo development is an important and unique period and is strictly controlled. This period includes a series of critical events that are regulated by multiple signal-transduction pathways, all of which are crucial in the establishment of a viable pregnancy. The p38 mitogen-activated protein kinase ( MAPK) signalling pathway is one of these pathways, and inhibition of its activity during preimplantation development has a deleterious effect. The molecular mechanisms underlying the deleterious effects of p38 MAPK suppression in early embryo development remain unknown. To investigate of the effect of p38 MAPK inhibition on late preimplantation stages in detail, we cultured 2-cell stage embryos in the presence of SB203580 for 48 h and analysed the 8-cell, morula, and blastocyst stages. We determined that prolonged inhibition of the p38 MAPK altered the expression levels of Glut1 and Glut4, decreased glucose uptake during the 8-cell to blastocyst transition, changed the expression levels of transcripts which will be important to lineage commitment, including Oct4/Pou5f1, Nanog, Sox2, and Gata6, and increased cell death in 8-16 cell stage embryos onwards. Strikingly, while the expression levels of Nanog, Gata6 and Oct4/Pou5f1 mRNAs were significantly decreased, Sox2 mRNA was increased in SB203580-treated blastocysts. Taken together, our results provide important insight into the biological processes controlled by the p38 MAPK pathway and its critical role during preimplantation development.en_US
dc.description.sponsorshipAkdeniz Universityen_US
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştırma Kurumu (TUBITAK)en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPreimplantationen_US
dc.subjectMouseen_US
dc.subjectP38 MAPKen_US
dc.subjectGLUTen_US
dc.subjectLineage Segregationen_US
dc.titleThe p38 MAPK signalling pathway is required for glucose metabolism, lineage specification and embryo survival during mouse preimplantation developmenten_US
dc.typearticleen_US
dc.relation.ispartofMechanisms of Developmenten_US
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalıen_US
dc.authorid0000-0001-6781-9983en_US
dc.identifier.volume138en_US
dc.identifier.startpage375en_US
dc.identifier.endpage398en_US
dc.relation.eceu-repo/grantAgreement/TUBITAK/SOBAG/113S160en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1016/j.mod.2015.05.002en_US
dc.identifier.wosqualityQ4en_US
dc.identifier.scopusqualityQ1en_US


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