Pregabalin attenuates carrageenan induced acute inflammation in rats by inhibiting proinflammatory cytokine levels
AuthorKılıç, Fatma Sultan
MetadataShow full item record
CitationKılıç, F., Kaygısız, B., Aydın, Ş., Yıldırım, C., Karımkhan, H. ve Öner, S. (2018). Pregabalin attenuates carrageenan induced acute inflammation in rats by inhibiting proinflammatory cytokine levels. Eurasian Journal of Medicine, 50(3) 156-159. https://dx.doi.org/10.5152/eurasianjmed.2018.17261
Objective: Pregabalin (PGB) is a compound used in the treatment of epilepsy, anxiety, and neuropathic pain. Experimental data also indicate that PGB can reduce inflammatory pain. We aimed to investigate the anti-inflammatory effects of PGB on carrageenan (CAR)-induced paw edema and its effects on tumor necrosis factor-alpha (TNF-alpha) and interleukine-1 beta (IL-1 beta) acting as acute phase cytokines in inflammation, and antiinflammatory cytokine IL-10, in rats. Materials and Methods: Single doses of PGB 30, 50, and 100 mg/kg and indomethacin (INDO) 5 mg/kg in the treatment groups and saline in the control group were injected once intraperitoneally prior to administration of 100 mu l of 1% CAR into the right hind paw of the rats. The paw thickness was measured using gauge calipers (Vernier calipers) before (0 hour) and every hour afterwards for 6 hours following the inflammation induction. The cytokine levels in the blood serum obtained intracardiacally were determined after 6 hours using the enzyme-linked immunosorbent assay method. p < 0.05 was considered statistically significant. Results: There was no significant difference between the 0 and 6th hour considering the paw thickness in all groups, except in the CAR group. CAR significantly increased the paw thickness at 6 hours compared to the 0 hour. All doses of PGB and INDO significantly reduced the paw thickness after 6 hours compared to the CAR group. The TNF-alpha and IL-1 beta levels in the PGB and INDO groups were comparable to the control group, whereas in the CAR group, these levels were increased. The IL-10 level was enhanced in the PGB 50 mg/kg and INDO groups. Conclusion: It was observed that all doses of PGB exerted anti-inflammatory-like effects comparable to INDO, supported by their effects on the levels of cytokines.