dc.contributor.author | Konishi, Masanori | |
dc.contributor.author | Erdem, Sultan Sibel | |
dc.contributor.author | Weissleder, Ralph | |
dc.contributor.author | Lichtman, Andrew | |
dc.contributor.author | McCarthy, Jason | |
dc.contributor.author | Libby, Peter | |
dc.date.accessioned | 10.07.201910:49:13 | |
dc.date.accessioned | 2019-07-10T19:51:09Z | |
dc.date.available | 10.07.201910:49:13 | |
dc.date.available | 2019-07-10T19:51:09Z | |
dc.date.issued | 2015 | en_US |
dc.identifier.citation | Konishi, M., Erdem, S. S., Weissleder, R., Lichtman, A., McCarthy, J. ve Libby, P. (2015). Imaging Granzyme B activity assesses immune-mediated myocarditis. Circulation Research, 117(6), 502-512. https://dx.doi.org/10.1161/CIRCRESAHA.115.306364 | en_US |
dc.identifier.issn | 0009-7330 | |
dc.identifier.issn | 1524-4571 | |
dc.identifier.uri | https://dx.doi.org/10.1161/CIRCRESAHA.115.306364 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/2158 | |
dc.description | WOS: 000360142000006 | en_US |
dc.description | PubMed ID: 26199323 | en_US |
dc.description.abstract | Rationale: The development of molecular imaging approaches that assess specific immunopathologic mechanisms can advance the study of myocarditis. Objective: This study validates a novel molecular imaging tool that enables the in vivo visualization of granzyme B activity, a major effector of cytotoxic CD8(+) T lymphocytes. Methods and Results: We synthesized and optimized a fluorogenic substrate capable of reporting on granzyme B activity and examined its specificity ex vivo in mice hearts with experimental cytotoxic CD8(+) T lymphocyte-mediated myocarditis using fluorescence reflectance imaging, validated by histological examination. In vivo experiments localized granzyme B activity in hearts with acute myocarditis monitored by fluorescent molecular tomography in conjunction with coregistered computed tomography imaging. A model anti-inflammatory intervention (dexamethasone administration) in vivo reduced granzyme B activity (vehicle versus dexamethasone: 504263 versus 194 +/- 77 fluorescence intensities in hearts; P=0.002). Conclusions: Molecular imaging of granzyme B activity can visualize T cell-mediated myocardial injury and monitor the response to an anti-inflammatory intervention. | en_US |
dc.description.sponsorship | National Institutes of Health National Heart, Lung, and Blood Institute contract [HHSN268201000044C, R01HL121363-01]; National Institutes of Health; Translational Program of Excellence in Nanotechnology; Japan Society for the Promotion of Science; Strategic Young Researcher Overseas Visits Program for Accelerating Brain Circulation; Uehara Memorial Foundation | en_US |
dc.description.sponsorship | This study was supported, in part, by National Institutes of Health National Heart, Lung, and Blood Institute contract HHSN268201000044C and R01HL121363-01, Grant-in-Aid for Scientific Research from the National Institutes of Health; Translational Program of Excellence in Nanotechnology, Japan Society for the Promotion of Science; Strategic Young Researcher Overseas Visits Program for Accelerating Brain Circulation, and The Uehara Memorial Foundation; Research Fellowship. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Lippincott Williams and Wilkins | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Dexamethasone | en_US |
dc.subject | Granzyme | en_US |
dc.subject | Immunology | en_US |
dc.subject | Molecular Imaging | en_US |
dc.subject | Myocarditis | en_US |
dc.title | Imaging Granzyme B activity assesses immune-mediated myocarditis | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Circulation Research | en_US |
dc.department | İstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı | en_US |
dc.identifier.volume | 117 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.startpage | 502 | en_US |
dc.identifier.endpage | 512 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1161/CIRCRESAHA.115.306364 | en_US |
dc.identifier.wosquality | Q1 | en_US |
dc.identifier.scopusquality | Q1 | en_US |