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dc.contributor.authorKaval Oğuz, Elif
dc.contributor.authorÖztürk, Gürkan
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:51:05Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:51:05Z
dc.date.issued2019en_US
dc.identifier.citationKaval Oğuz, E. ve Öztürk, G. (2019). An in vitro model for conditioning lesion effect. Cellular and Molecular Neurobiology, 39(1), 61-71. https://dx.doi.org/10.1007/s10571-018-0633-2en_US
dc.identifier.issn0272-4340
dc.identifier.issn1573-6830
dc.identifier.urihttps://dx.doi.org/10.1007/s10571-018-0633-2
dc.identifier.urihttps://hdl.handle.net/20.500.12511/2143
dc.descriptionWOS: 000455226200004en_US
dc.descriptionPubMed ID: 30415355en_US
dc.description.abstractAxons of a peripheral nerve grow faster after an axotomy if it attains a prior injury a few days earlier. This is called conditioning lesion effect (CLE) and very much valued since it may provide new insights into neuron biology and axonal regeneration. There are established in vivo experimental paradigms to study CLE, however, there is a need to have an in vitro conditioning technique where CLE occurs in a maximally controlled environment. Mouse primary sensory neurons were isolated from lumbar 4-5 dorsal root ganglia and incubated at 37 degrees C on a silicon-coated watch glass that prevents cell attachment. After this conditioning period they were transferred to laminin coated culture dishes. Similar cultures were set up with freshly isolated neurons from control animals and from the animals that received a sciatic nerve cut 3days earlier. All preparations were placed on a live cell imaging microscopy providing physiological conditions and photographed for 48h. Axonal regeneration and neuronal survival was assessed. During the conditioning incubation period neurons remained in suspended aggregates and did not grow axons. The regeneration rate of the in vitro conditioned neurons was much higher than the in vivo conditioned and control preparations during the first day of normal incubation. However, higher regeneration rates were compromised by progressive substantial neuronal death in both types of conditioned cultures but not in the control preparations. By using neutralizing antibodies, we demonstrated that activity of endogenous leukemia inhibitory factor is essential for induction of CLE in this model.en_US
dc.description.sponsorshipYuzuncu Yil University, Directorate of Scientific Research Projectsen_US
dc.description.sponsorshipThis study was supported by Yuzuncu Yl University, Directorate of Scientific Research Projects.en_US
dc.language.isoengen_US
dc.publisherSpringer/Plenum Publisheren_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectConditioningen_US
dc.subjectIn Vitroen_US
dc.subjectNeuron Cultureen_US
dc.subjectAxon Regenerationen_US
dc.subjectDegenerationen_US
dc.subjectLeukemia Inhibitory Factoren_US
dc.subjectLIFen_US
dc.titleAn in vitro model for conditioning lesion effecten_US
dc.typearticleen_US
dc.relation.ispartofCellular and Molecular Neurobiologyen_US
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalıen_US
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)en_US
dc.authorid0000-0003-0352-1947en_US
dc.identifier.volume39en_US
dc.identifier.issue1en_US
dc.identifier.startpage61en_US
dc.identifier.endpage71en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1007/s10571-018-0633-2en_US
dc.identifier.wosqualityQ2en_US
dc.identifier.scopusqualityQ1en_US


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