dc.contributor.author | Ayşit Altuncu, Neşe | |
dc.contributor.author | Ulusoy, Canan | |
dc.contributor.author | Öztürk, Gürkan | |
dc.contributor.author | Tüzün, Erdem | |
dc.date.accessioned | 10.07.201910:49:13 | |
dc.date.accessioned | 2019-07-10T19:50:25Z | |
dc.date.available | 10.07.201910:49:13 | |
dc.date.available | 2019-07-10T19:50:25Z | |
dc.date.issued | 2018 | en_US |
dc.identifier.citation | Ayşit Altuncu, N., Ulusoy, C., Öztürk, G. ve Tüzün, E. (2018). Effect of LGI1 antibody-positive IgG on hippocampal neuron survival: A preliminary study. Neuroreport, 29(11), 932-938. https://dx.doi.org/10.1097/WNR.0000000000001055 | en_US |
dc.identifier.issn | 0959-4965 | |
dc.identifier.issn | 1473-558X | |
dc.identifier.uri | https://dx.doi.org/10.1097/WNR.0000000000001055 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/1979 | |
dc.description | WOS: 000437757600009 | en_US |
dc.description | PubMed ID: 29771820 | en_US |
dc.description.abstract | Anti-leucine-rich glioma inactivated 1 (anti-LGI1) encephalitis is one of the most frequently encountered forms of autoimmune encephalitis. Many patients with anti-LGI1 encephalitis develop permanent hippocampal neuron loss and chronic neuropsychiatric symptoms, suggesting that LGI antibodies (Ab) might have a neurotoxic action. To investigate this hypothesis, purified serum IgG of three patients with anti-LGI1 encephalitis and six healthy controls were incubated with cultured primary hippocampal neurons obtained from newborn mice. Nontreated cells were used as controls. The viability of IgG-treated neurons was evaluated by propidium iodide staining. Apoptotic mechanisms were assessed by JC-1 assay and mRNA expression level measurement of apoptosis-related genes using real-time PCR. The effect of IgG treatment on calcium influx was analyzed by fluo-4 calcium imaging. LGI1-Ab+ IgG increased the number of propidium iodide positive neurons, reduced mitochondrial membrane potentials, upregulated caspase-3 and Bax mRNA expression levels and downregulated Bcl-2 mRNA expression levels of neurons. LGI1-Ab+ IgG-treated neurons showed lower calcium staining than healthy controls IgG-treated and non-IgG-treated neurons. Our results indicate a neurotoxic role of LGI1-Ab. This neurotoxicity is likely mediated through induction of apoptosis and reduction of calcium currents. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Lippincott Williams & Wilkins | en_US |
dc.rights | info:eu-repo/semantics/embargoedAccess | en_US |
dc.subject | Antibody | en_US |
dc.subject | Apoptosis | en_US |
dc.subject | Autoimmunity | en_US |
dc.subject | Calcium Influx | en_US |
dc.subject | Leucine-Rich Glioma-Inactivated 1 | en_US |
dc.title | Effect of LGI1 antibody-positive IgG on hippocampal neuron survival: A preliminary study | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Neuroreport | en_US |
dc.department | İstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı | en_US |
dc.authorid | 0000-0003-0352-1947 | en_US |
dc.identifier.volume | 29 | en_US |
dc.identifier.issue | 11 | en_US |
dc.identifier.startpage | 932 | en_US |
dc.identifier.endpage | 938 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1097/WNR.0000000000001055 | en_US |
dc.identifier.wosquality | Q4 | en_US |
dc.identifier.scopusquality | Q3 | en_US |