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dc.contributor.authorKarabulut, Seda
dc.contributor.authorDemiroğlu Zergeroğlu, Asuman
dc.contributor.authorYılmaz, Elif
dc.contributor.authorKutlu, Pelin
dc.contributor.authorKeskin, Ilknur
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:50:21Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:50:21Z
dc.date.issued2018en_US
dc.identifier.citationKarabulut, S., Demiroğlu Zergeroğlu, A., Yılmaz, E., Kutlu, P. ve Keskin, I. (2018). Effects of human sperm cryopreservation on apoptotic markers in normozoospermic and non-normozoospermic patients. Zygote, 26(4), 308-313. https://dx.doi.org/10.1017/S0967199418000254en_US
dc.identifier.issn0967-1994
dc.identifier.issn1469-8730
dc.identifier.urihttps://dx.doi.org/10.1017/S0967199418000254
dc.identifier.urihttps://hdl.handle.net/20.500.12511/1958
dc.descriptionWOS: 000448811000008en_US
dc.descriptionPubMed ID: 30220260en_US
dc.description.abstractThe negative effects of cryopreservation on sperm parameters are well documented but little information is known about molecular basis of the process. The aim of the present study was to investigate the possible effects of sperm cryopreservation on main apoptotic signs including DNA fragmentation and caspase-3 activation and to determine if these effects vary according to sperm parameters. Sperm samples of 72 patients were cryopreserved. The patients were sub-grouped as normozoospermic or non-normozoospermic patients according to their semen parameters. DNA fragmentation rates and caspase-3 activation levels were analyzed before and after cryopreservation in both groups. Mean DNA fragmentation rate was increased significantly from 23.98% in neat semen samples to 27.34% after cryopreservation (P = 0.03). DNA fragmentation rates were slightly higher in non-normozoospermic patients compared with the normozoospermic patients in both the neat semen and after cryopreservation (23.25 and 24.71% vs. 26.32 and 28.36%, respectively) although the difference obtained were not statistically significant. An increasing trend for caspase-3 activations (0.093 vs. 0.116) was observed after cryopreservation but the differences were not statistically significant. Caspase-3 activation was found to be slightly higher in non-normozoospermic patients both in the neat semen and after cryopreservation compared with the normozoospermic patients but the differences were not statistically significant. Caspase-3 expression was also shown using immunocytochemistry in both fresh ejaculated sperm and thawed sperm after cryopreservation but at different localizations. The cryopreservation process had detrimental effects on sperm quality but the quality of the sperm samples was not adversely effective for the apoptotic markers including DNA fragmentation and caspase-3 activation patterns. In fact, it was the cryopreservation process itself that adversely effected the above apoptotic markers and apoptosis. It was concluded therefore that sperm cell cryopreservation triggers apoptosis after thawing and this process adversely affects semen parameters.en_US
dc.language.isoengen_US
dc.publisherCambridge University Pressen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectApoptosisen_US
dc.subjectCaspase-3en_US
dc.subjectCryopreservationen_US
dc.subjectDNA fragmentationen_US
dc.subjectSpermen_US
dc.titleEffects of human sperm cryopreservation on apoptotic markers in normozoospermic and non-normozoospermic patientsen_US
dc.typearticleen_US
dc.relation.journalZygoteen_US
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalıen_US
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)en_US
dc.authorid0000-0003-3302-5004en_US
dc.authorid0000-0002-7059-1884en_US
dc.identifier.volume26en_US
dc.identifier.issue4en_US
dc.identifier.startpage308en_US
dc.identifier.endpage313en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1017/S0967199418000254en_US


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