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dc.contributor.authorKaraarslan, Numan
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorÖzbek, Hanefi
dc.contributor.authorYaşar Şirin, Duygu
dc.contributor.authorKaplan, Necati
dc.contributor.authorAkyuva, Yener
dc.contributor.authorGönültaş, Aylin
dc.contributor.authorAteş, Özkan
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:49:54Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:49:54Z
dc.date.issued2019en_US
dc.identifier.citationKaraaslan, N., Yılmaz, İ., Özbek, H., Yaşar Şirin, D., Kaplan, N., Akyuva, Y. … Ateş, Ö. (2019). Are specific gene expressions of extracellular matrix and nucleus pulposus affected by primary cell cultures prepared from intact or degenerative intervertebral disc tissues? Turkish Neurosurgery, 29(1), 43-52. https://dx.doi.org/10.5137/1019-5149.JTN.22210-17.2en_US
dc.identifier.issn1019-5149
dc.identifier.urihttps://dx.doi.org/10.5137/1019-5149.JTN.22210-17.2
dc.identifier.urihttps://hdl.handle.net/20.500.12511/1812
dc.descriptionWOS: 000454722700007en_US
dc.descriptionPubMed ID: 29484626en_US
dc.description.abstractAIM: To determine the gene expression patterns of nucleus pulposus (NP) in cell cultures obtained from degenerated or intact tissues. MATERIAL and METHODS: Whereas 12 of the cases were diagnosed with lumbar disc herniation and had undergone lumbar microdiscectomy, 12 cases had undergone traumatic intervertebral discectomy and corpectomy, along with discectomy after spinal trauma. NP-specific markers and gene expressions of the reagents of the extracellular matrix in the experimental setup were tested at the 0th, 24th, and 48th hours by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Visual evaluations were simultaneously made in all samples using invert and fluorescence microscopy. Vitality and proliferation analyses were evaluated by UV spectrophotometer. As a method of statistical evaluation, Spearman was used for categorical variants, and the Pearson correlation was used for variants with numerical and plain distribution. RESULTS: No association was found either between the tissue type and times (r=0.000; p=1.000) or between the region that the tissue was obtained from and hypoxia transcription factor-1 alpha (HIF-1 alpha) gene expression (r=0.098; p=0.245). There was no correlation between cell proliferation and chondroadherin (CHAD) expression or between type II collagen (COL2A1) and CHAD gene expressions. It was found that CHAD and HIF-1 alpha gene expressions and HIF-1 alpha and COL2A1 gene expressions affected cell proliferation. CONCLUSION: Cell culture setups are of paramount importance because they may influence the pattern of changes in the gene expressions of the cells used in these setups.en_US
dc.language.isoengen_US
dc.publisherTurkish Neurosurgical Societyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAnnulus Fibrosusen_US
dc.subjectDegenerative Disc Diseaseen_US
dc.subjectNucleus Pulposusen_US
dc.subjectSpecific Markeren_US
dc.subjectPharmaco-Molecular Therapyen_US
dc.subjectHuman Primary Cell Cultureen_US
dc.titleAre specific gene expressions of extracellular matrix and nucleus pulposus affected by primary cell cultures prepared from intact or degenerative intervertebral disc tissues?en_US
dc.typearticleen_US
dc.relation.ispartofTurkish Neurosurgeryen_US
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Farmakoloji Ana Bilim Dalıen_US
dc.authorid0000-0003-2003-6337en_US
dc.authorid0000-0002-8084-7855en_US
dc.identifier.volume29en_US
dc.identifier.issue1en_US
dc.identifier.startpage43en_US
dc.identifier.endpage52en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.5137/1019-5149.JTN.22210-17.2en_US
dc.identifier.wosqualityQ4en_US
dc.identifier.scopusqualityQ3en_US


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