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dc.contributor.authorKeskin, İlknur
dc.contributor.authorSütçü, Mustafa
dc.contributor.authorEren, Hilal
dc.contributor.authorKeskin, Mustafa
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:37:42Z
dc.date.available10.07.201910:49:14
dc.date.available2019-07-10T19:37:42Z
dc.date.issued2017en_US
dc.identifier.citationKeskin, İ., Sütçü, M., Eren, H. ve Keskin, M. (2017). Exposure to tumescent solution significantly increases phosphorylation of perilipin in adipocytes. Aesthetic Surgery Journal, 37(2), 239-245. https://dx.doi.org/10.1093/asj/sjw156en_US
dc.identifier.issn1527-330X
dc.identifier.issn1090-820X
dc.identifier.urihttps://hdl.handle.net/20.500.12511/1455
dc.identifier.urihttps://dx.doi.org/10.1093/asj/sjw156
dc.description.abstractBACKGROUND: Lidocaine and epinephrine could potentially decrease adipocyte viability, but these effects have not been substantiated. The phosphorylation status of perilipin in adipocytes may be predictive of cell viability. Perilipin coats lipid droplets and restricts access of lipases; phospho-perilipin lacks this protective function.OBJECTIVES: The authors investigated the effects of tumescent solution containing lidocaine and epinephrine on the phosphorylation status of perilipin in adipocytes.METHODS: In this in vitro study, lipoaspirates were collected before and after tumescence from 15 women who underwent abdominoplasty. Fat samples were fixed, sectioned, and stained for histologic and immunohistochemical analyses. Relative phosphorylation of perilipin was inferred from pixel intensities of immunostained adipocytes observed with confocal microscopy.RESULTS: For adipocytes collected before tumescent infiltration, 10.08% of total perilipin was phosphorylated. In contrast, 30.62% of total perilipin was phosphorylated for adipocytes collected from tumescent tissue (P < .01).CONCLUSIONS: The tumescent technique increases the relative phosphorylation of perilipin in adipocytes, making these cells more vulnerable to lipolysis. Tumescent solution applied for analgesia or hemostasis of the donor site should contain the lowest possible concentrations of lidocaine and epinephrine. LEVEL OF EVIDENCE 5.en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBackgrounden_US
dc.subjectObjectivesen_US
dc.subjectMethodsen_US
dc.subjectResultsen_US
dc.subjectConclusionsen_US
dc.titleExposure to tumescent solution significantly increases phosphorylation of perilipin in adipocytesen_US
dc.typearticleen_US
dc.relation.journalAesthetic Surgery Journalen_US
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)en_US
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalıen_US
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Plastik, Rekonstrüktif ve Estetik Cerrahi Ana Bilim Dalıen_US
dc.authorid0000-0002-7059-1884en_US
dc.authorid0000-0001-5106-0159en_US
dc.authorid0000-0003-2066-7454en_US
dc.identifier.volume37en_US
dc.identifier.issue2en_US
dc.identifier.startpage239en_US
dc.identifier.endpage245en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.doi10.1093/asj/sjw156en_US


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