dc.contributor.author | Abdallah, Anas | |
dc.contributor.author | Tekin, Abdurrahim | |
dc.contributor.author | Öztanır, Mustafa Namık | |
dc.contributor.author | Süsgün, Seda | |
dc.contributor.author | Yabacı, Ayşegül | |
dc.contributor.author | Çınar, İrfan | |
dc.contributor.author | Can, Engin | |
dc.contributor.author | Tokar, Sadık | |
dc.contributor.author | Akbaş, Fahri | |
dc.contributor.author | Seyithanoğlu, Mehmet Hakan | |
dc.date.accessioned | 2023-09-21T11:19:38Z | |
dc.date.available | 2023-09-21T11:19:38Z | |
dc.date.issued | 2023 | en_US |
dc.identifier.citation | Abdallah, A., Tekin, A., Öztanır, M. N., Süsgün, S., Yabacı, A., Çınar, İ. ... Seyithanoğlu, M. H. (2023). Effects of methylprednisolone in the treatment of spinal cord injuries by evaluation of microRNA-21: An experimental study. Journal of Neurological Surgery, Part A: Central European Neurosurgery, 84(3), 240-246. https://doi.org/10.1055/s-0042-1743552 | en_US |
dc.identifier.issn | 2193-6315 | |
dc.identifier.issn | 2193-6323 | |
dc.identifier.uri | https://doi.org/10.1055/s-0042-1743552 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/11481 | |
dc.description.abstract | Background and Study Aims Spinal cord injury (SCI) is one of the most complicated pathologies that affect active young males. miR-21 primarily regulates several cellular processes. We aimed to elucidate the regulatory role of miR-21 and test methylprednisolone as a disease-modifying agent on experimental SCI tissues. Methods A total of 36 8- to 10-week-old adult female Sprague-Dawley rats weighing 250 to 300 g were used. Animals were randomly divided into six groups. Except for groups 1 and 4, the spinal trauma model was applied to all animal groups using the clipping method. In groups 3 and 6, methylprednisolone was given. For real-time polymerase chain reaction (PCR) investigations, rats in groups 1, 2, and 3 were reoperated on after the first postoperative day, whereas those in groups 4, 5, and 6 were reoperated on after postoperative day 7 and spinal cord samples from the laminectomy area were removed for gene expression analysis. Relative gene expression of miR-21, Gfap , Vim , Stat3 , Faslg , Pten , Bax , Bcl2 , Cox2 , and Il6 were determined with quantitative reverse transcription (qRT) PCR. Results In group 3, the miR-21 expression significantly increased compared with groups 1 and 2. When compared with group 3, a decrease in miR-21 expression was observed in group 6 ( p < 0.05). When compared with group 4, group 6 had lower levels of Gfap , Pten , Stat3 , and Bax ( p < 0.05). Conclusions miR-21 supports the beneficial aspects of the body's healing mechanisms following SCI. In the acute phase, the use of methylprednisolone increases miR-21 expression in the early period of trauma. Methylprednisolone increases some astrogliosis and inflammation biomarkers' levels; however, it did not affect the apoptotic biomarkers. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Georg Thieme Verlag | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Spinal Cord Injury | en_US |
dc.subject | miR-21 | en_US |
dc.subject | Methylprednisolone | en_US |
dc.subject | Real-Time PCR | en_US |
dc.title | Effects of methylprednisolone in the treatment of spinal cord injuries by evaluation of microRNA-21: An experimental study | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Journal of Neurological Surgery, Part A: Central European Neurosurgery | en_US |
dc.department | İstanbul Medipol Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Beyin ve Sinir Cerrahisi Ana Bilim Dalı | en_US |
dc.authorid | 0000-0001-5401-1547 | en_US |
dc.identifier.volume | 84 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.startpage | 240 | en_US |
dc.identifier.endpage | 246 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1055/s-0042-1743552 | en_US |
dc.institutionauthor | Tekin, Abdurrahim | |
dc.identifier.wosquality | Q4 | en_US |
dc.identifier.wos | 000783930100001 | en_US |
dc.identifier.scopus | 2-s2.0-85128930852 | en_US |
dc.identifier.pmid | 35439827 | en_US |
dc.identifier.scopusquality | Q3 | en_US |