dc.contributor.author | Kolukırık, Mustafa | |
dc.contributor.author | Yılmaz, Mesut | |
dc.contributor.author | İnce, Orhan | |
dc.contributor.author | Ketre, Canan | |
dc.contributor.author | Tosun İstanbullu, Ayşe | |
dc.contributor.author | İnce Kasapgil, Bahar | |
dc.date.accessioned | 10.07.201910:49:13 | |
dc.date.accessioned | 2019-07-10T19:36:22Z | |
dc.date.available | 10.07.201910:49:14 | |
dc.date.available | 2019-07-10T19:36:22Z | |
dc.date.issued | 2016 | en_US |
dc.identifier.citation | Kolukırık, M., Yılmaz, M., İnce, O., Ketre, C., Tosun İstanbullu, A. ve İnce Kasapgil, B. (2016). Development of a fast and low-cost qPCR assay for diagnosis of acute gas pharyngitis. Annals of Clinical Microbiology and Antimicrobials, 15(1), 2-6. https://dx.doi.org/10.1186/s12941-016-0162-0 | en_US |
dc.identifier.issn | 1476-0711 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/1144 | |
dc.identifier.uri | https://dx.doi.org/10.1186/s12941-016-0162-0 | |
dc.description.abstract | Background: Group A streptococci (GAS) are the most common bacterial cause of acute pharyngitis and account for 15-30 % of cases of acute pharyngitis in children and 5-10 % of cases in adults. In this study, a real-time quantitative PCR (qPCR) based GAS detection assay in pharyngeal swab specimens was developed. Methods: The qPCR assay was compared with the gold standard bacterial culture and a rapid antigen detection test (RADT) to evaluate its clinical performance in 687 patients. The analytical sensitivity of the assay was 240 cfu/swab. Forty-five different potential cross-reacting organisms did not react with the test. Four different laboratories for the reproducibility studies were in 100 % (60/60) agreement for the contrived GAS positive and negative swab samples. Results: The relative sensitivities of the RADT and the qPCR test were 55.9 and 100 %; and the relative specificities were 100 and 96.3 %, respectively. Duration of the total assay for 24 samples including pre-analytical processing and analysis changed between 42 and 55 min depending on the type of qPCR instrument used. A simple DNA extraction method and a low qPCR volume made the developed assay an economical alternative for the GAS detection. Conclusion: We showed that the developed qPCR test is rapid, cheap, sensitive and specific and therefore can be used to replace both antigen detection and culture for diagnosis of acute GAS pharyngitis. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | BioMed Central Ltd. | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Acute Pharyngitis | en_US |
dc.subject | Group A Streptococci | en_US |
dc.subject | QPCR | en_US |
dc.subject | Rapid Antigen Detection Test | en_US |
dc.title | Development of a fast and low-cost qPCR assay for diagnosis of acute gas pharyngitis | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Annals of Clinical Microbiology and Antimicrobials | en_US |
dc.department | İstanbul Medipol Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Ana Bilim Dalı | en_US |
dc.authorid | 0000-0001-8022-7325 | en_US |
dc.identifier.volume | 15 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.startpage | 2 | en_US |
dc.identifier.endpage | 6 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1186/s12941-016-0162-0 | en_US |
dc.identifier.wosquality | Q2 | en_US |
dc.identifier.scopusquality | Q1 | en_US |