Sağlık Hizmetleri Meslek Yüksekokulu
https://hdl.handle.net/20.500.12511/4200
Vocational School of Health Services2024-03-28T12:00:49ZOptimization of inulin extraction from chicory roots and an ultrafiltration application to obtain purified inulin and hydrolyzed fructooligosaccharides
https://hdl.handle.net/20.500.12511/12250
Optimization of inulin extraction from chicory roots and an ultrafiltration application to obtain purified inulin and hydrolyzed fructooligosaccharides
Sağcan, Nihan; Sağcan, Hasan; Bozkurt, Fatih; Bulut Güneş, Ayşe Nur; Fakir, Hüseyin; Dertli, Enes; Sağdıç, Osman
Inulin and fructooligosaccharides (FOS) are prominent functional components in the food industry due to prebiotic and other pharmaceutical properties. Inulin is a storage polysaccharide in various plants. FOS are naturally present in various plants and can be obtained by partial hydrolysis of inulin. In this study, ground and sieved chicory roots (Cichorium intybus L.) were used as starting material for inulin extraction under optimized conditions determined by Response Surface Methodology (RSM) with a Box-Behnken design. Optimum inulin extraction conditions from chicory roots were; temperature of 90 °C, extraction time of 30 min., and liquid-to-solid (LS) ratio of 10:1 mL/g. Inulin extract was further hydrolyzed to FOS by enzymatic or acid treatment, separately. Purification of inulin extract and FOS hydrolysate was performed by ultrafiltration with a 10 kDa membrane under the pressure of 2 bar with continuous stirring. As a result, inulin and FOS were obtained at 90% and 76% purity, respectively.
2024-01-01T00:00:00ZrTMS reduces delta and increases theta oscillations in Alzheimer's disease: A visual-evoked and event-related potentials study
https://hdl.handle.net/20.500.12511/12210
rTMS reduces delta and increases theta oscillations in Alzheimer's disease: A visual-evoked and event-related potentials study
Velioğlu, Halil Aziz; Düdükçü, Esra Zeynep; Hanoğlu, Lütfü; Güntekin, Bahar; Aktürk, Tuba; Yuluğ, Burak
Background: Repetitive transcranial magnetic stimulation (rTMS) has emerged as a promising alternative therapy for Alzheimer's disease (AD) due to its ability to modulate neural networks and enhance cognitive function. This treatment offers the unique advantage of enabling real-time monitoring of immediate cognitive effects and dynamic brain changes through electroencephalography (EEG).Objective: This study focused on exploring the effects of left parietal rTMS stimulation on visual-evoked potentials (VEP) and visual event-related potentials (VERP) in AD patients.Methods: Sixteen AD patients were recruited for this longitudinal study. EEG data were collected within a Faraday cage both pre- and post-rTMS to evaluate its impact on potentials.Results: Significant alterations were found in both VEP and VERP oscillations. Specifically, delta power in VEP decreased, while theta power in VERP increased post-rTMS, indicating a modulation of brain activities.Discussion: These findings confirm the positive modulatory impact of rTMS on brain activities in AD, evidenced by improved cognitive scores. They align with previous studies highlighting the potential of rTMS in managing hyperexcitability and oscillatory disturbances in the AD cortex.Conclusion: Cognitive improvements post-rTMS endorse its potential as a promising neuromodulatory treatment for cognitive enhancement in AD, thereby providing critical insights into the neurophysiological anomalies in AD and possible therapeutic avenues.
2024-01-01T00:00:00ZPoor reactivity of posterior electroencephalographic alpha rhythms during the eyes open condition in patients with dementia due to Parkinson's disease
https://hdl.handle.net/20.500.12511/12093
Poor reactivity of posterior electroencephalographic alpha rhythms during the eyes open condition in patients with dementia due to Parkinson's disease
Babiloni, Claudio; Noce, Giuseppe; Tucci, Federico; Jakhar, Dharmendra; Ferri, Raffaele; Panerai, Simonetta; Catania, Valentina; Soricelli, Andrea; del Percio, Claudio
Here, we hypothesized that the reactivity of posterior resting-state electroencephalographic (rsEEG) alpha rhythms during the transition from eyes-closed to -open condition might be lower in patients with Parkinson's disease dementia (PDD) than in patients with Alzheimer's disease dementia (ADD). A Eurasian database provided clinical-demographic-rsEEG datasets in 73 PDD patients, 35 ADD patients, and 25 matched cognitively unimpaired (Healthy) persons. The eLORETA freeware was used to estimate cortical rsEEG sources. Results showed substantial (greater than −10%) reduction (reactivity) in the posterior alpha source activities from the eyes-closed to the eyes-open condition in 88% of the Healthy seniors, 57% of the ADD patients, and only 35% of the PDD patients. In these alpha-reactive participants, there was lower reactivity in the parietal alpha source activities in the PDD group than in the healthy control seniors and the ADD patients. These results suggest that PDD patients show poor reactivity of mechanisms desynchronizing posterior rsEEG alpha rhythms in response to visual inputs. That neurophysiological biomarker may provide an endpoint for (non) pharmacological interventions for improving vigilance regulation in those patients.
2024-01-01T00:00:00ZSynthesis, structural investigations, DNA/BSA interactions, molecular docking studies, and anticancer activity of a new 1,4-disubstituted 1,2,3-triazole derivative
https://hdl.handle.net/20.500.12511/11583
Synthesis, structural investigations, DNA/BSA interactions, molecular docking studies, and anticancer activity of a new 1,4-disubstituted 1,2,3-triazole derivative
Göktürk, Tolga; Sakallı Çetin, Esin; Hökelek, Tuncer; Pekel, Hanife; Şensoy, Özge; Aksu, Ebru Nur; Güp, Ramazan
We report herein a new 1,2,3-triazole derivative, namely, 4-(( 1-( 3,4-dichlorophenyl)-1H-1,2,3- triazol- 4-yl)methoxy)-2-hydroxybenzaldehyde, which was synthesized by copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC). The structure of the compound was analyzed using Fourier transform infrared spectroscopy (FTIR), H-1 NMR, C-13 NMR, UV-vis, and elemental analyses. Moreover, X- ray crystallography studies demonstrated that the compound adapted a monoclinic crystal system with the P2(1)/c space group. The dominant interactions formed in the crystal packing were found to be hydrogen bonding and van der Waals interactions according to Hirshfeld surface (HS) analysis. The volume of the crystal voids and the percentage of free spaces in the unit cell were calculated as 152.10 A(3) and 9.80%, respectively. The evaluation of energy frameworks showed that stabilization of the compound was dominated by dispersion energy contributions. Both in vitro and in silico investigations on the DNA/bovine serum albumin (BSA) binding activity of the compound showed that the CT-DNA binding activity of the compound was mediated via intercalation and BSA binding activity was mediated via both polar and hydrophobic interactions. The anticancer activity of the compound was also tested by the 3-(4,5-dimethylthiazol2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay using human cell lines including MDA-MB-231, LNCaP, Caco-2, and HEK293. The compound exhibited more cytotoxic activity than cisplatin and etoposide on Caco-2 cancer cell lines with an IC50 value of 16.63 +/- 0.27 mu M after 48 h. Annexin V suggests the induction of cell death by apoptosis. Compound 3 significantly increased the loss of mitochondrial membrane potential (MMP) levels in Caco-2 cells, and the reactive oxygen species (ROS) assay proved that compound 3 could induce apoptosis by ROS generation.
2023-01-01T00:00:00Z