Biyomedikal Cihaz Teknolojisi
https://hdl.handle.net/20.500.12511/218
Biomedical Equipment Tecnology2024-03-28T23:49:18ZSimulating compressive three-dimensional structured illumination microscopy
https://hdl.handle.net/20.500.12511/1393
Simulating compressive three-dimensional structured illumination microscopy
Özgürün, Baturay; Çetin, Müjdat
Three-dimensional structured illumination microscopy (3D-SIM) requires at least fifteen raw images to extend the spatial resolution to the half of the diffraction limit in both axial and lateral directions. This requirement can cause motion artifacts in a reconstructed super-resolution image. Therefore, reducing the acquisition duration of raw images is of interest. Here, we propose a novel method, which applies compressed sensing (CS) to 3D-SIM and enables faster raw image acquisitions. CS allows us to record a scene with a photomultiplier tube, which is much faster than a camera. Furthermore CS can recover the scene from a small number of measurements. The combination of these two facts leads to significant reduction of the overall acquisition time. In preliminary experiments, a computer-generated ground truth image is utilized to simulate the proposed method, and the spatial resolution of an image produced by the proposed method is compared with conventional optical microscopy and 3D-SIM.
2019-01-01T00:00:00ZA simple and low cost structured illumination microscopy using a pico-projector
https://hdl.handle.net/20.500.12511/3797
A simple and low cost structured illumination microscopy using a pico-projector
Özgürün, Baturay
Here, development of a low-cost structured illumination microscopy (SIM) based on a pico-projector is presented. The pico-projector consists of independent red, green and blue LEDs that remove need for an external illumination source. Moreover, display element of the pico-projector serves as a pattern generating spatial light modulator. A simple lens group is employed to couple light from the projector to an epi-illumination port of a commercial microscope system. 2D sub SIM images are acquired and synthesized to surpass the diffraction limit using 40x (0.75 NA) objective. Resolution of the reconstructed SIM images is verified with a dye-and-object object and a fixed cell sample.
Conference on Single Molecule Spectroscopy and Superresolution Imaging XI -- JAN 27-28, 2018 -- San Francisco, CA; WOS: 000456502700004
2018-01-01T00:00:00Z