Makale KoleksiyonuArticle Collectionhttps://hdl.handle.net/20.500.12511/45062024-03-29T15:18:58Z2024-03-29T15:18:58ZOptimization of inulin extraction from chicory roots and an ultrafiltration application to obtain purified inulin and hydrolyzed fructooligosaccharidesSağcan, NihanSağcan, HasanBozkurt, FatihBulut Güneş, Ayşe NurFakir, HüseyinDertli, EnesSağdıç, Osmanhttps://hdl.handle.net/20.500.12511/122502024-02-07T12:37:03Z2024-01-01T00:00:00ZOptimization of inulin extraction from chicory roots and an ultrafiltration application to obtain purified inulin and hydrolyzed fructooligosaccharides
Sağcan, Nihan; Sağcan, Hasan; Bozkurt, Fatih; Bulut Güneş, Ayşe Nur; Fakir, Hüseyin; Dertli, Enes; Sağdıç, Osman
Inulin and fructooligosaccharides (FOS) are prominent functional components in the food industry due to prebiotic and other pharmaceutical properties. Inulin is a storage polysaccharide in various plants. FOS are naturally present in various plants and can be obtained by partial hydrolysis of inulin. In this study, ground and sieved chicory roots (Cichorium intybus L.) were used as starting material for inulin extraction under optimized conditions determined by Response Surface Methodology (RSM) with a Box-Behnken design. Optimum inulin extraction conditions from chicory roots were; temperature of 90 °C, extraction time of 30 min., and liquid-to-solid (LS) ratio of 10:1 mL/g. Inulin extract was further hydrolyzed to FOS by enzymatic or acid treatment, separately. Purification of inulin extract and FOS hydrolysate was performed by ultrafiltration with a 10 kDa membrane under the pressure of 2 bar with continuous stirring. As a result, inulin and FOS were obtained at 90% and 76% purity, respectively.
2024-01-01T00:00:00ZAnalysis of the prechiasmatic sulcus in chiari malformation type IÖzalp, HakanÖzgüral, OnurAlpergin, Baran Canİnceoğlu, AyşenurÖzalp, SibelArmağan, ErcanUçar, HadiceBeger, Orhanhttps://hdl.handle.net/20.500.12511/112842023-08-08T11:03:03Z2023-01-01T00:00:00ZAnalysis of the prechiasmatic sulcus in chiari malformation type I
Özalp, Hakan; Özgüral, Onur; Alpergin, Baran Can; İnceoğlu, Ayşenur; Özalp, Sibel; Armağan, Ercan; Uçar, Hadice; Beger, Orhan
OBJECTIVE: To assess morphologic features of the pre-chiasmatic sulcus (PS) in Chiari malformation type I (CM-I).-METHODS: Computed tomography images were ob-tained in patients with CM-1 (mean age: 21.26 & PLUSMN; 16.46 years; sex: 25 females and 17 males) and 58 healthy control subjects (mean age: 28.12 & PLUSMN; 22.60 years; sex: 29 females and 29 males). The following parameters were measured to determine the anatomy of the PS: planum length, sulcal length, sulcal angle (SA), and interoptic distance. Consid-ering sulcal length and SA measurements, 4 types of PS were identified: narrow-steep-groove (NSG), narrow-flat -groove (NFG), wide-steep-groove, and wide-flat-groove.-RESULTS: SA was greater in the CM-I group (32.80 & PLUSMN; 18.21 & DEG;) compared with the control group (23.05 & PLUSMN; 14.53 & DEG;) (P = 0.004), but the other parameters were similar in both groups. Distribution ranking of PS types was as follows: NFG (31%) > NSG (26.20%) > wide-steep-groove (23.80%) > wide-flat-groove (19%) in the CM-I group; wide-flat-groove (37.90%) > wide-steep-groove (20.70%) = NFG (20.70%) = NSG (20.70%) in the control group. Distribution rate of PS types relative to both groups showed that the classification system was not affected by CM-I (P = 0.226).-CONCLUSIONS: SA value was approximately 25% greater in the CM-I group than in the control group; thus, CM-I patients had more vertical groove. The percentage of narrow-type (NSG D NFG) PS in the CM-I group was higher than in the control group.
2023-01-01T00:00:00ZSurface characterization, electrochemical properties and in vitro biological properties of Zn-deposited TiO2 nanotube surfacesDurdu, SalihCihan, GizemYalçın, EmineÇavuşoğlu, KültiginAltınkök, AtılganSağcan, HasanYurtsever, İlknurUsta, Metinhttps://hdl.handle.net/20.500.12511/112752023-08-17T11:31:06Z2023-01-01T00:00:00ZSurface characterization, electrochemical properties and in vitro biological properties of Zn-deposited TiO2 nanotube surfaces
Durdu, Salih; Cihan, Gizem; Yalçın, Emine; Çavuşoğlu, Kültigin; Altınkök, Atılgan; Sağcan, Hasan; Yurtsever, İlknur; Usta, Metin
In this work, to improve antibacterial, biocompatible and bioactive properties of commercial pure titanium (cp-Ti) for implant applications, the Zn-deposited nanotube surfaces were fabricated on cp-Ti by using combined anodic oxidation (AO) and physical vapor deposition (PVD-TE) methods. Homogenous elemental distributions were observed through all surfaces. Moreover, Zn-deposited surfaces exhibited hydrophobic character while bare Ti surfaces were hydrophilic. Due to the biodegradable behavior of Zn on the nanotube surface, Zn-deposited nanotube surfaces showed higher corrosion current density than bare cp-Ti surface in SBF conditions as expected. In vitro biological properties such as cell viability, ALP activity, protein adsorption, hemolytic activity and antibacterial activity for Gram-positive and Gram-negative bacteria of all surfaces were investigated in detail. Cell viability, ALP activity and antibacterial properties of Zn-deposited nanotube surfaces were significantly improved with respect to bare cp-Ti. Moreover, hemolytic activity and protein adsorption of Zn-deposited nanotube surfaces were decreased. According to these results; a bioactive, biocompatible and antibacterial Zn-deposited nanotube surfaces produced on cp-Ti by using combined AO and PVD techniques can have potential for orthopedic and dental implant applications.
2023-01-01T00:00:00ZThe optimal method to measure polyamines in serum by using HPLC fluorescence detectorÇakan, OrhanHacıhasanoğlu Çakmak, NezihaBayramoğlu, FeyzaYiğitbaşı, Türkanhttps://hdl.handle.net/20.500.12511/106612023-03-22T12:22:57Z2023-01-01T00:00:00ZThe optimal method to measure polyamines in serum by using HPLC fluorescence detector
Çakan, Orhan; Hacıhasanoğlu Çakmak, Neziha; Bayramoğlu, Feyza; Yiğitbaşı, Türkan
Measurement of polyamines (putrescine, spermidine and spermine) is important in the monitoring of many metabolic events, as well as in cancer prognosis. However, the lack of method standardization and validation for the measurement of poly-amines is a major obstacle. In this study, sample preparation, gradient program, chromatography column, sample concentration studies were attempted and an analysis method was developed for the detection of polyamines in serum by using HPLC fluorescence detector in order to reduce the difficulties experienced with the existing methodologies. This method was validated analytically in accordance with EMA and FDA’s guidelines. In this study, Waters Nova-Pak C18 A chromatography column with 3.9 mm, 150 mm and 4 µm specifications was used for the first time. Acetonitrile was used instead of methanol. Thanks to the newly developed gradient, peak purity and sufficient peak separation were ensured and accurate, sensitive, reliable and reproducible method was developed and validated.
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