Demirci, FatihKaradağ, Ayşe EsraBiltekin, Sevde NurDemirci, Betül2021-12-162021-12-162021Demirci, F., Karadağ, A. E., Biltekin, S. N. ve Demirci, B. (2021). In vitro ACE2 and 5-LOX enzyme inhibition by menthol and three different mint essential oils. Natural Product Communications, 16(11). https://dx.doi.org/10.1177/1934578X2110550141934-578X1555-9475https://dx.doi.org/10.1177/1934578X211055014https://hdl.handle.net/20.500.12511/8682Mentha arvensis L., M. citrata L., and M. spicata L. (family Lamiaceae) essential oils, and their characteristic constituent, menthol, were evaluated in vitro for angiotensin converting enzyme 2 (ACE2) and 5-lipoxygenase (5-LOX) enzyme inhibitory activity. The chemical compositions of M. arvensis, M. citrata, and M. spicata essential oils were analysed both by GC-FID, and GC/MS; 82.0%, 38.1%, and 0.4% menthol were identified, respectively. M. spicata essential oil contained 88.2% carvone as its major component. The enzyme inhibitory activities of the essential oils were evaluated using a fluorometric multiplate based enzyme inhibition kit; the ACE2 inhibitions produced by M. arvensis, M. citrata, and M. spicata essential oils were 33%, 22%, and 73%, while the 5-LOX inhibitions were 84%, 79%, and 70%, respectively. In addition, menthol also showed remarkable ACE2 inhibition of 99.8%, whereas the 5-LOX inhibition was 79.9%. As a result, menthol and the three different mint essential oils may have antiviral potential applications against coronaviruses due to their ACE2 enzyme inhibition and anti-inflammatory features. However, further in vivo studies are needed to confirm the safety and efficacy.enAttribution-NonCommercial 4.0 Internationalinfo:eu-repo/semantics/openAccessMenthaEssential OilACE2LOXMentholArticle161110.1177/1934578X211055014Q4Q3