Elmas, BahriKaracan, MehmetDervişoğlu, PınarKösecik, Mustafaİşgüven, Şükriye PınarBal, Ceylan10.07.20192019-07-1010.07.20192019-07-102017Elmas, B., Karacan, M., Dervişoğlu, P., Kösecik, M., İşgüven, Ş. P. ve Bal, C. (2017). Dynamic thiol/disulphide homeostasis as a novel indicator of oxidative stress in obese children and its relationship with inflammatory-cardiovascular markers. Anatolian Journal of Cardiology, 18(5), 361-369. https://dx.doi.org/10.14744/AnatolJCardiol.2017.77402149-22632149-2271https://hdl.handle.net/20.500.12511/1320https://dx.doi.org/10.14744/AnatolJCardiol.2017.7740Objective: Childhood obesity is an important cause of cardiovascular risk with chronic inflammation. Oxidative stress may contribute to the pathogenesis of obesity-related cardiovascular pathologies. We aimed to evaluate thiol/disulphide homeostasis as a novel and sensitive marker of oxidative stress and to evaluate its relationship with some inflammatory and cardiovascular markers in obese children. Methods: In this case-controlled study, 65 children with exogenous obesity and 64 healthy children, as a control group, were included. In both groups, thiol/disulphide homeostasis parameters and inflammatory (white blood cells, platelets, mean corpuscular volume, neutrophil/lympho-cyte ratio, and high-sensitivity C-reactive protein) and cardiovascular (epicardial adipose tissue thickness and left ventricular mass index) markers were studied. Correlation analyses of thiol/disulphide homeostasis parameters with body mass index standard deviation scores (BMI SDS) and inflammatory and cardiovascular markers were performed. Receiver-operating characteristic analysis was performed to determine the sensitivity, specificity, and optimal cut-off values of thiol/disulphide homeostasis parameters. Results: Native thiol, total thiol, and native thiol/total thiol ratios (antioxidant parameters) were lower (p<0.05) and disulphide/native thiol and disulphide/total thiol ratios (oxidant parameters) were higher in the obese group than in the control group (p<0.01). A positive correlation of oxidant parameters with BMI SDS and inflammatory markers was found. However, a negative correlation of antioxidant parameters with BMI SDS and inflammatory markers was found. The specificities of disulphide/native thiol and disulphide/total thiol ratios were higher in the obese group. Conclusion: The impairment in thiol/disulphide homeostasis, which is indicative of oxidative stress, is associated with inflammation in obesity. In addition, cardiovascular involvement may also contribute to this impairment.eninfo:eu-repo/semantics/openAccessCardiovascular RiskChildrenInflammationObesityOxidative StressThiol/Disulphide HomeostasisArticle18536136910.14744/AnatolJCardiol.2017.7740Q4Q3