Arslan, FerhatMert, AliSili, UluhanTabak, FehmiÖztürk, RecepÖzaras, Reşat10.07.20192019-07-1010.07.20192019-07-102014Arslan, F., Mert, A., Sili, U., Tabak, F., Öztürk, R. ve Özaras, R. (2014). Comparison of cellular immunity in patients with chronic hepatitis B, inactive hepatitis B surface antigen carriers and spontaneously recovered individuals. Hepato-Gastroenterology, 61(136), 2326-2329.0172-6390https://hdl.handle.net/20.500.12511/2612WOS: 000346326500036PubMed ID: 25699376Different clinical outcomes of acute HBV infection have been partially explained by individual differences in immune response. In this study We investigated interferon gamma (IFN-gamma) secretion of peripheral blood mononuclear cells (PBMC) in vitro against specific (Hepatitis B core antigen; recombinant HBcAg) and non-specific (CMV, EBV, Influenza peptide pool; CTL CFF peptide pool "plus") antigens using enzyme linked immunospot (ELISPOT) assay in 7 patients with chronic hepatitis B (CHB group), 8 inactive carriers of HBV (carrier group) and 8 subjects who spontaneously recovered from acute HBV infection as detected by anti-HBs positivity (immune group). Phytohemaglutinin served as the positive test control. Response against recombinant HBcAg was 88 +/- 35, 50 +/- 110, 105 +/- 150 spot forming cell (SFC) /10(5) PBMC, in CHB, carrier and immune groups, respectively. HBeAg-specific T-cell response was slightly higher in the immune group; however, statistically there was no significant difference between the groups. Assessment of cellular immunity by IFN-gamma ELISPOT was not sufficient to explain the various outcomes of HBV infection such as resolution, chronicity and carriership.eninfo:eu-repo/semantics/closedAccessChronic Hepatitis BELISPOT AssayInterferon GammaArticle611362326232910.5754/hge13891Q4Q2