Yashar, MeltemBaşarır, Kerem EdipTanrıverdi, Elif SerenCelep, SelçukSirekbasan, LeylaRakıcı, ErvaEjder, NebahatMüsellim, EdaÇopur Çiçek, AyşegülYılmaz, Mesut2024-06-132024-06-132024Yashar, M., Başarır, K. E., Tanrıverdi, E. S., Celep, S., Sirekbasan, L., Rakıcı, E. ... Yılmaz, M. (2024). Stenotrophomonas maltophilia outbreak originating from a pull-out faucet in a pediatric intensive care unit in Turkey: Insights from clinical records and molecular typing. American Journal of Infection Control, 52(5), 605-610. http://dx.doi.org/10.1016/j.ajic.2023.11.0180196-65531527-3296http://dx.doi.org/10.1016/j.ajic.2023.11.018https://hdl.handle.net/20.500.12511/12641Background: Nosocomial Stenotrophomonas maltophilia-related cases are rising and pose a threat to immunocompromised patients. Twelve patients from our pediatric intensive care unit (PICU) presented with S maltophilia-associated bloodstream infection. Methods: This outbreak investigation includes 12 patients from PICU between the ages of 2 months and 4 years (mean 16 months, 7 male). To identify the origin, samples from all possible sources throughout the hospital were collected and ran through DNA isolation and Pulse Field Gel Electrophoresis. Results: 120 samples were collected during the outbreak. 31 samples (26%) were positive for S maltophilia. 30 S maltophilia isolates were analyzed, 10 different genotypes were identified. Clustering isolates were grouped into 3 different clusters (tolerance and optimization 1.0, cutoff 90%). The largest cluster was genotype 1, which included 19 isolates, those belong to patients’ samples and a sample from a pull-out faucet inside the PICU. The Pull-out faucet was the origin of the bloodstream infection. Discussion: Pull-out faucets allow biofilm production, due its structure. Pulse Field Gel Electrophoresis identifies the transmission dynamics of the outbreak, with its high discriminatory power. Conclusions: Water sources should be monitored on a regular basis. Pull-out faucets enable bacterial overgrowth; therefore, we recommend water surveillance during outbreak investigations.eninfo:eu-repo/semantics/openAccessFaucet AeratorGenotypeImmunocompromised PatientsOutbreak-InvestigationStenotrophomonas Maltophilia BacteremiaArticle52560561010.1016/j.ajic.2023.11.018Q10012339151000012-s2.0-8517981036438043636Q1